Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats
1 The Charles A. Dana Research Institute and the Harvard-Thorndike Laboratory; Cardiovascular Division, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston 02215; and 2 Department of Pathology, The Holy Family Hospital and Medical Center, Methuen, Massac...
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Veröffentlicht in: | Journal of applied physiology (1985) 2002-01, Vol.92 (1), p.288-296 |
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Sprache: | eng |
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Zusammenfassung: | 1 The Charles A. Dana Research Institute and the
Harvard-Thorndike Laboratory; Cardiovascular Division, Department
of Medicine, Beth Israel Deaconess Medical Center and Harvard
Medical School, Boston 02215; and 2 Department of Pathology,
The Holy Family Hospital and Medical Center, Methuen, Massachusetts
01884
Massive loss of cardiac myocytes after
myocardial infarction (MI) is a common cause of heart failure. The
present study was designed to investigate the improvement of cardiac
function in MI rats after embryonic stem (ES) cell transplantation. MI
in rats was induced by ligation of the left anterior descending
coronary artery. Cultured ES cells used for cell transplantation were
transfected with the marker green fluorescent protein (GFP). Animals in
the treated group received intramyocardial injection of ES cells in injured myocardium. Compared with the MI control group injected with an
equivalent volume of the cell-free medium, cardiac function in ES
cell-implanted MI animals was significantly improved 6 wk after cell
transplantation. The characteristic phenotype of engrafted ES cells was
identified in implanted myocardium by strong positive staining to
sarcomeric -actin, cardiac -myosin heavy chain, and troponin I. GFP-positive cells in myocardium sectioned from MI hearts confirmed the
survival and differentiation of engrafted cells. In addition, single
cells isolated from cell-transplanted MI hearts showed rod-shaped
GFP-positive myocytes with typical striations. The present data
demonstrate that ES cell transplantation is a feasible and novel
approach to improve ventricular function in infarcted failing hearts.
cell transplantation; myocardial infarction |
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ISSN: | 8750-7587 1522-1601 |
DOI: | 10.1152/jappl.2002.92.1.288 |