The microRNA-producing enzyme Dicer1 is essential for zebrafish development

MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but...

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Veröffentlicht in:	Nature genetics 2003-11, Vol.35 (3), p.217-218
Hauptverfasser:	Plasterk, Ronald H A, Wienholds, Erno, Koudijs, Marco J, van Eeden, Freek J M, Cuppen, Edwin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell physiology Danio rerio DEAD-box RNA Helicases Dicer1 gene Endoribonucleases - genetics Endoribonucleases - physiology Fundamental and applied biological sciences. Psychology Gene Silencing Genetic aspects Inactivation Marine MicroRNAs - biosynthesis miRNA Molecular and cellular biology Mutation Physiological aspects Ribonuclease III RNA RNA Helicases - genetics RNA Helicases - physiology Structure Vertebrates Zebra fish Zebrafish - embryology
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Beschreibung
Zusammenfassung:	MicroRNAs (miRNAs) are produced by the Dicer1 enzyme; the role of Dicer1 in vertebrate development is unknown. Here we report target-selected inactivation of the dicer1 gene in zebrafish. We observed an initial build-up of miRNA levels, produced by maternal Dicer1, in homozygous dicer1 mutants, but miRNA accumulation stopped after a few days. This resulted in developmental arrest around day 10. These results indicate that miRNA-producing Dicer1 is essential for vertebrate development.
ISSN:	1061-4036 1546-1718
DOI:	10.1038/ng1251