Disruption of protein-protein interactions: design of a synthetic receptor that blocks the binding of cytochrome c to cytochrome c peroxidase

Synthetic receptor 1 has been found via fluorescence titration to compete effectively with cytochrome c peroxidase for binding cytochrome c (Cc), forming 1:1 Cc:1 complex with a binding constant of (3 +/- 1) x 10(8) M-1, and to disrupt Cc: Apaf-1 complex, a key adduct in apoptosis.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2001-09 (17), p.1580-1581
Hauptverfasser:	Wei, Y, McLendon, G L, Hamilton, A D, Case, M A, Purring, C B, Lin, Q, Park, H S, Lee, C S, Yu, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis - drug effects Apoptotic Protease-Activating Factor 1 Binding, Competitive - drug effects Carrier Proteins - chemical synthesis Carrier Proteins - metabolism Carrier Proteins - pharmacology Cytochrome c Group - antagonists & inhibitors Cytochrome c Group - metabolism Cytochrome-c Peroxidase - antagonists & inhibitors Cytochrome-c Peroxidase - metabolism Protein Binding - drug effects Protein Conformation Proteins - antagonists & inhibitors Proteins - metabolism Spectrometry, Fluorescence Thermodynamics
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Beschreibung
Zusammenfassung:	Synthetic receptor 1 has been found via fluorescence titration to compete effectively with cytochrome c peroxidase for binding cytochrome c (Cc), forming 1:1 Cc:1 complex with a binding constant of (3 +/- 1) x 10(8) M-1, and to disrupt Cc: Apaf-1 complex, a key adduct in apoptosis.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/b104142h