Regulation of prostaglandin biosynthesis in dispersed choriodecidual cells in culture

We have evaluated the prostaglandin (PG) production and PG biosynthetic gene expression in a choriodecidual dispersed cell culture system. Cells dispersed from human choriodecidual membranes by dispase and trypsin digestion were evaluated after 1, 3, 5 and 7 days of culture for basal and tumour necr...

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Veröffentlicht in:	Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2001-11, Vol.65 (5-6), p.247-251
Hauptverfasser:	Marvin, K.W., Mitchell, M.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cells, Cultured Chorion - cytology Chorion - drug effects Chorion - metabolism Cyclooxygenase 1 Cyclooxygenase 2 Decidua - cytology Decidua - drug effects Decidua - metabolism Dinoprostone - biosynthesis Female Gene Expression Regulation Humans Isoenzymes - drug effects Isoenzymes - genetics Isoenzymes - metabolism Membrane Proteins Phospholipases A - drug effects Phospholipases A - genetics Phospholipases A - metabolism Phospholipases A2 Pregnancy Prostaglandin-Endoperoxide Synthases - drug effects Prostaglandin-Endoperoxide Synthases - genetics Prostaglandin-Endoperoxide Synthases - metabolism RNA, Messenger - drug effects Tumor Necrosis Factor-alpha - pharmacology
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Zusammenfassung:	We have evaluated the prostaglandin (PG) production and PG biosynthetic gene expression in a choriodecidual dispersed cell culture system. Cells dispersed from human choriodecidual membranes by dispase and trypsin digestion were evaluated after 1, 3, 5 and 7 days of culture for basal and tumour necrosis factor α (F- α) stimulated PGE2 production. The highest rates of production (P
ISSN:	0952-3278 1532-2823
DOI:	10.1054/plef.2001.0321