Phloroglucinol: novel synthesis and role of the magnesium cation on its binding with human serum albumin (HSA) using a biochromatographic approach based on Langmuir isotherms

In this paper, a new and efficient method for synthesis of phloroglucinol with an overall yield of 60% was described. As well, the phloroglucinol association on an immobilized human serum albumin (HSA) column was analyzed in biochromatography by the determination of its Langmuir distribution isotherms. The role of the magnesium cation Mg 2+ on the phloroglucinol–HSA binding process was as well analyzed. The results showed that in the Mg 2+ concentration range (0.7–2 mM) (including its biological concentration range, i.e. 0.75–0.90 mM), increasing the Mg 2+ concentration increased the fraction of free phloroglucinol (not linked with HSA) and thus its biological effect.