Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples
This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with fer...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2003-07, Vol.75 (14), p.3316-3321 |
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| creator | Lim, Tae-Kyu Ohta, Hiroko Matsunaga, Tadashi |
| description | This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody−antigen complexes were separated from free Fc-conjugated IgG antibody (Fc−IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200−2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results. |
| doi_str_mv | 10.1021/ac020749n |
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The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody−antigen complexes were separated from free Fc-conjugated IgG antibody (Fc−IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200−2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac020749n</identifier><identifier>PMID: 14570179</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Blood ; Chemistry ; Electrochemical methods ; Electrochemistry - instrumentation ; Exact sciences and technology ; Ferrous Compounds - chemistry ; Histamine - blood ; Humans ; Hypersensitivity - blood ; Immunoassay ; Immunoassay - instrumentation ; Immunoglobulin G - analysis ; Metallocenes ; Microcomputers ; Miscellaneous ; Nanotechnology ; Pollen - immunology ; Polymethyl Methacrylate</subject><ispartof>Analytical chemistry (Washington), 2003-07, Vol.75 (14), p.3316-3321</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><rights>Copyright American Chemical Society Jul 15, 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a608t-705baec13f67053c96ceed2e71c98a1e2875f02122b9c68a8ae2118f6fbf37833</citedby><cites>FETCH-LOGICAL-a608t-705baec13f67053c96ceed2e71c98a1e2875f02122b9c68a8ae2118f6fbf37833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac020749n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac020749n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27080,27928,27929,56742,56792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16166114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14570179$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Tae-Kyu</creatorcontrib><creatorcontrib>Ohta, Hiroko</creatorcontrib><creatorcontrib>Matsunaga, Tadashi</creatorcontrib><title>Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody−antigen complexes were separated from free Fc-conjugated IgG antibody (Fc−IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200−2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.</description><subject>Analytical chemistry</subject><subject>Blood</subject><subject>Chemistry</subject><subject>Electrochemical methods</subject><subject>Electrochemistry - instrumentation</subject><subject>Exact sciences and technology</subject><subject>Ferrous Compounds - chemistry</subject><subject>Histamine - blood</subject><subject>Humans</subject><subject>Hypersensitivity - blood</subject><subject>Immunoassay</subject><subject>Immunoassay - instrumentation</subject><subject>Immunoglobulin G - analysis</subject><subject>Metallocenes</subject><subject>Microcomputers</subject><subject>Miscellaneous</subject><subject>Nanotechnology</subject><subject>Pollen - immunology</subject><subject>Polymethyl Methacrylate</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCgT-ALCSQOAQ8TmInx3b7KXVVxC7iaDnesTbFiYOdiO6ZP46rXXUlOHCypXn8emYeQt4A-wSMw2dtGGeyqPtnZAYlZ5moKv6czBhjecYlY0fkOMZ7xgAYiJfkCIpSMpD1jPxetCZ4q5vQGj3imt712XzTDtlqOyC9cGjG4M0Gu1R29NL5X_Sm66be6xj1li63ccSOWh_ouEF6jmN60Pqeekuv2zjqru2RfkWHOqbwtqffN94hPXPer-lSd4PD-Iq8sNpFfL0_T8i3y4vV_Dq7vbu6mZ_eZlqwaswkKxuNBnIr0jU3tTCIa44STF1pQF7J0qZ1cN7URlS60sgBKitsY3NZ5fkJ-bDLHYL_OWEcVddGg87pHv0UlQRe87oU_wWh5rwoCkjgu7_Aez-FPg2hOMhKypw_fvtxB6VFxxjQqiG0nQ5bBUw9-lNP_hL7dh84NR2uD-ReWALe7wEdkxEbdG_aeOAECAFQJC7bcUkCPjzVdfihhMxlqVZflup8UZyVVyumFodcbeJhiH8b_APS6708</recordid><startdate>20030715</startdate><enddate>20030715</enddate><creator>Lim, Tae-Kyu</creator><creator>Ohta, Hiroko</creator><creator>Matsunaga, Tadashi</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030715</creationdate><title>Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples</title><author>Lim, Tae-Kyu ; Ohta, Hiroko ; Matsunaga, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a608t-705baec13f67053c96ceed2e71c98a1e2875f02122b9c68a8ae2118f6fbf37833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analytical chemistry</topic><topic>Blood</topic><topic>Chemistry</topic><topic>Electrochemical methods</topic><topic>Electrochemistry - instrumentation</topic><topic>Exact sciences and technology</topic><topic>Ferrous Compounds - chemistry</topic><topic>Histamine - blood</topic><topic>Humans</topic><topic>Hypersensitivity - blood</topic><topic>Immunoassay</topic><topic>Immunoassay - instrumentation</topic><topic>Immunoglobulin G - analysis</topic><topic>Metallocenes</topic><topic>Microcomputers</topic><topic>Miscellaneous</topic><topic>Nanotechnology</topic><topic>Pollen - immunology</topic><topic>Polymethyl Methacrylate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Tae-Kyu</creatorcontrib><creatorcontrib>Ohta, Hiroko</creatorcontrib><creatorcontrib>Matsunaga, Tadashi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Tae-Kyu</au><au>Ohta, Hiroko</au><au>Matsunaga, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2003-07-15</date><risdate>2003</risdate><volume>75</volume><issue>14</issue><spage>3316</spage><epage>3321</epage><pages>3316-3321</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody−antigen complexes were separated from free Fc-conjugated IgG antibody (Fc−IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200−2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>14570179</pmid><doi>10.1021/ac020749n</doi><tpages>6</tpages></addata></record> |
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| subjects | Analytical chemistry Blood Chemistry Electrochemical methods Electrochemistry - instrumentation Exact sciences and technology Ferrous Compounds - chemistry Histamine - blood Humans Hypersensitivity - blood Immunoassay Immunoassay - instrumentation Immunoglobulin G - analysis Metallocenes Microcomputers Miscellaneous Nanotechnology Pollen - immunology Polymethyl Methacrylate |
| title | Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples |
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