Microfabricated On-Chip-Type Electrochemical Flow Immunoassay System for the Detection of Histamine Released in Whole Blood Samples
This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with fer...
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Veröffentlicht in: | Analytical chemistry (Washington) 2003-07, Vol.75 (14), p.3316-3321 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | This paper describes an on-chip-type electrochemical flow immunoassay system with a multichanneled matrix column. The multichanneled matrix column was functionally coated with cation-exchange resin and used for separation of proteins. Antihistamine immunoglobulin G (IgG) antibody conjugated with ferrocenemonocarboxylic acid (Fc) was also prepared and used as a novel analytical reagent. Antibody−antigen complexes were separated from free Fc-conjugated IgG antibody (Fc−IgG) on the basis of differences in isoelectric point (pI) using the multichanneled matrix column coated with cation-exchange resin. The assay yields a good relationship between current and histamine concentration in the range of 200−2000 ng/mL. This simple technique enables the assay of histamine released in whole blood within 2 min. Furthermore, a good correlation was found between the response of the electrochemical immunoassay described in this paper and the conventional RIA (radioimmunoassay). This on-chip-type electrochemical flow immunoassay requires only minute quantities of whole blood samples and generates highly reproducible results. |
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ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac020749n |