Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

Directed Evolution of an Amine Oxidase Possessing both Broad Substrate Specificity and High Enantioselectivity

Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)‐α‐methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see sc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie International Edition 2003-10, Vol.42 (39), p.4807-4810
Hauptverfasser: Carr, Reuben, Alexeeva, Marina, Enright, Alexis, Eve, Tom S. C., Dawson, Michael J., Turner, Nicholas J.
Format: Artikel
Sprache:eng
Schlagworte:
Amine Oxidase (Copper-Containing) - chemistry
Amine Oxidase (Copper-Containing) - genetics
Amine Oxidase (Copper-Containing) - metabolism
amines
Amines - chemistry
deracemization
directed evolution
enzymes
Evolution, Molecular
Mutation
Stereoisomerism
Structure-Activity Relationship
Substrate Specificity
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)‐α‐methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200352100