A dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis

IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tube...

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Veröffentlicht in:	Indian journal of experimental biology 2001-10, Vol.39 (10), p.984-988
Hauptverfasser:	Sumi, M G, Mathai, A, Reuben, S, Radhakrishnan, V V, Sasikumar, S, Jayapal, V, Felix, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Antibodies, Bacterial - blood Antigens, Bacterial - immunology Child Child, Preschool Diagnosis, Differential Electrophoresis, Polyacrylamide Gel Female Humans Immunoassay Immunoblotting - methods Male Middle Aged Mycobacterium tuberculosis - immunology Sensitivity and Specificity Sputum - microbiology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - microbiology
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Zusammenfassung:	IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.
ISSN:	0019-5189