A cytochemical method for visualising blood vessels selectively in semithin LR white sections

Traditional cytochemical techniques for illustrating blood vessels are usually unsuitable for use in high resolution, 0.35 microm-thick LR White sections due to the thinness of the tissue and, if collagenous, high background staining. We report that a modification of the periodic acid-thiocarbohydra...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of molecular histology 2001-07, Vol.33 (7), p.433-435
Hauptverfasser:	von Ruhland, C J, Newman, G R
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins Blood Blood Vessels - chemistry Blood Vessels - metabolism Humans Hydrazines - chemistry Microtomy Periodic Acid - chemistry Periodic Acid-Schiff Reaction - methods Peritoneum - blood supply White people
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Traditional cytochemical techniques for illustrating blood vessels are usually unsuitable for use in high resolution, 0.35 microm-thick LR White sections due to the thinness of the tissue and, if collagenous, high background staining. We report that a modification of the periodic acid-thiocarbohydrazide-silver proteinate-silver enhancement method (PATCH-SP-SE) selectively visualises, with low background, even the smallest blood vessels in semithin LR White sections of highly collagenous tissues such as human parietal peritoneum.
ISSN:	0018-2214 1567-2379 1567-2387
DOI:	10.1023/A:1013732112957