Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies
Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene pr...
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| Veröffentlicht in: | Journal of leukocyte biology 2000-07, Vol.68 (1), p.151-157 |
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| container_title | Journal of leukocyte biology |
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| creator | Shakhov, Alexander N. Lyakhov, Ilya G. Tumanov, Alexei V. Rubtsov, Anatoly V. Drutskaya, Ludmila N. Marino, Michael W. Nedospasov, Sergei A. |
| description | Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling. |
| doi_str_mv | 10.1189/jlb.68.1.151 |
| format | Article |
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To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.68.1.151</identifier><identifier>PMID: 10914503</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>Animals ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - genetics ; Chemokines - biosynthesis ; Chemokines - genetics ; DNA, Complementary - genetics ; Expressed Sequence Tags ; gene arrays ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Group II Phospholipases A2 ; knockout mice ; Lymphoid Tissue - pathology ; lymphotoxin-^a ; Lymphotoxin-alpha - genetics ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neutrophils - metabolism ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Organ Specificity ; Pancreas - enzymology ; Phenotype ; Phospholipases A - biosynthesis ; Phospholipases A - genetics ; Phospholipases A - isolation & purification ; Receptors, Chemokine - biosynthesis ; Receptors, Chemokine - genetics ; Specific Pathogen-Free Organisms ; spleen ; Spleen - metabolism ; Spleen - pathology ; Subtraction Technique ; subtractive cloning ; Tumor Necrosis Factor-alpha - deficiency ; Tumor Necrosis Factor-alpha - genetics</subject><ispartof>Journal of leukocyte biology, 2000-07, Vol.68 (1), p.151-157</ispartof><rights>2000 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3901-459553e937fc75029e821c9e037856e6e54c081c1acb3b40bf92744e7016b0653</citedby><cites>FETCH-LOGICAL-c3901-459553e937fc75029e821c9e037856e6e54c081c1acb3b40bf92744e7016b0653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.68.1.151$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.68.1.151$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10914503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shakhov, Alexander N.</creatorcontrib><creatorcontrib>Lyakhov, Ilya G.</creatorcontrib><creatorcontrib>Tumanov, Alexei V.</creatorcontrib><creatorcontrib>Rubtsov, Anatoly V.</creatorcontrib><creatorcontrib>Drutskaya, Ludmila N.</creatorcontrib><creatorcontrib>Marino, Michael W.</creatorcontrib><creatorcontrib>Nedospasov, Sergei A.</creatorcontrib><title>Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.</description><subject>Animals</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Chemokines - biosynthesis</subject><subject>Chemokines - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>Expressed Sequence Tags</subject><subject>gene arrays</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation</subject><subject>Group II Phospholipases A2</subject><subject>knockout mice</subject><subject>Lymphoid Tissue - pathology</subject><subject>lymphotoxin-^a</subject><subject>Lymphotoxin-alpha - genetics</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Neutrophils - metabolism</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Organ Specificity</subject><subject>Pancreas - enzymology</subject><subject>Phenotype</subject><subject>Phospholipases A - biosynthesis</subject><subject>Phospholipases A - genetics</subject><subject>Phospholipases A - isolation & purification</subject><subject>Receptors, Chemokine - biosynthesis</subject><subject>Receptors, Chemokine - genetics</subject><subject>Specific Pathogen-Free Organisms</subject><subject>spleen</subject><subject>Spleen - metabolism</subject><subject>Spleen - pathology</subject><subject>Subtraction Technique</subject><subject>subtractive cloning</subject><subject>Tumor Necrosis Factor-alpha - deficiency</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQRi1ERbeFG2fkC5ya7Uwc2_ERKlqgq3IpZ8vxThpXTrLEuwr773GVCnGCw8gj-fl91sfYW4Q1Ym0uH2OzVvUa1yjxBVuhEXUhlBYv2Qp0hYWsAE7ZWUqPACBKBa_YKYLBSoJYsdsbGojvprENMQwP3O3y7nzHw8D3HXE3uHhMIfGx5fHY77pxP_7Kd27Y8vu7a76lNvhAQ570mp20LiZ683yesx_Xn--vvhSb7zdfrz5uCi8MYFFJI6UgI3TrtYTSUF2iNwRC11KRIll5qNGj841oKmhaU-qqIg2oGlBSnLMPizd_9eeB0t72IXmK0Q00HpLVWEoEVf8XRJ2dKJ_AiwX005jSRK3dTaF309Ei2KeWbW7ZqtqizS1n_N2z99D0tP0LXmrNAC7AHCId_ymz3zafYJG-X9504aGbw0Q29S7GHFHaeZ7_hP8GlfqStw</recordid><startdate>200007</startdate><enddate>200007</enddate><creator>Shakhov, Alexander N.</creator><creator>Lyakhov, Ilya G.</creator><creator>Tumanov, Alexei V.</creator><creator>Rubtsov, Anatoly V.</creator><creator>Drutskaya, Ludmila N.</creator><creator>Marino, Michael W.</creator><creator>Nedospasov, Sergei A.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200007</creationdate><title>Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies</title><author>Shakhov, Alexander N. ; Lyakhov, Ilya G. ; Tumanov, Alexei V. ; Rubtsov, Anatoly V. ; Drutskaya, Ludmila N. ; Marino, Michael W. ; Nedospasov, Sergei A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3901-459553e937fc75029e821c9e037856e6e54c081c1acb3b40bf92744e7016b0653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Chemokines - biosynthesis</topic><topic>Chemokines - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>Expressed Sequence Tags</topic><topic>gene arrays</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation</topic><topic>Group II Phospholipases A2</topic><topic>knockout mice</topic><topic>Lymphoid Tissue - pathology</topic><topic>lymphotoxin-^a</topic><topic>Lymphotoxin-alpha - genetics</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Neutrophils - metabolism</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Organ Specificity</topic><topic>Pancreas - enzymology</topic><topic>Phenotype</topic><topic>Phospholipases A - biosynthesis</topic><topic>Phospholipases A - genetics</topic><topic>Phospholipases A - isolation & purification</topic><topic>Receptors, Chemokine - biosynthesis</topic><topic>Receptors, Chemokine - genetics</topic><topic>Specific Pathogen-Free Organisms</topic><topic>spleen</topic><topic>Spleen - metabolism</topic><topic>Spleen - pathology</topic><topic>Subtraction Technique</topic><topic>subtractive cloning</topic><topic>Tumor Necrosis Factor-alpha - deficiency</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shakhov, Alexander N.</creatorcontrib><creatorcontrib>Lyakhov, Ilya G.</creatorcontrib><creatorcontrib>Tumanov, Alexei V.</creatorcontrib><creatorcontrib>Rubtsov, Anatoly V.</creatorcontrib><creatorcontrib>Drutskaya, Ludmila N.</creatorcontrib><creatorcontrib>Marino, Michael W.</creatorcontrib><creatorcontrib>Nedospasov, Sergei A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shakhov, Alexander N.</au><au>Lyakhov, Ilya G.</au><au>Tumanov, Alexei V.</au><au>Rubtsov, Anatoly V.</au><au>Drutskaya, Ludmila N.</au><au>Marino, Michael W.</au><au>Nedospasov, Sergei A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>2000-07</date><risdate>2000</risdate><volume>68</volume><issue>1</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>10914503</pmid><doi>10.1189/jlb.68.1.151</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals |
| subjects | Animals Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Chemokines - biosynthesis Chemokines - genetics DNA, Complementary - genetics Expressed Sequence Tags gene arrays Gene Expression Profiling - methods Gene Expression Regulation Group II Phospholipases A2 knockout mice Lymphoid Tissue - pathology lymphotoxin-^a Lymphotoxin-alpha - genetics Membrane Proteins - biosynthesis Membrane Proteins - genetics Mice Mice, Inbred C57BL Mice, Knockout Neutrophils - metabolism Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Organ Specificity Pancreas - enzymology Phenotype Phospholipases A - biosynthesis Phospholipases A - genetics Phospholipases A - isolation & purification Receptors, Chemokine - biosynthesis Receptors, Chemokine - genetics Specific Pathogen-Free Organisms spleen Spleen - metabolism Spleen - pathology Subtraction Technique subtractive cloning Tumor Necrosis Factor-alpha - deficiency Tumor Necrosis Factor-alpha - genetics |
| title | Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies |
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