Gene profiling approach in the analysis of lymphotoxin and TNF deficiencies

Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene pr...

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Veröffentlicht in:Journal of leukocyte biology 2000-07, Vol.68 (1), p.151-157
Hauptverfasser: Shakhov, Alexander N., Lyakhov, Ilya G., Tumanov, Alexei V., Rubtsov, Anatoly V., Drutskaya, Ludmila N., Marino, Michael W., Nedospasov, Sergei A.
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Sprache:eng
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Zusammenfassung:Mice with combined lymphotoxin‐α (LTα) and tumor necrosis factor (TNF) deficiencies show defects in the structure of peripheral lymphoid organs such as spleen, lymph nodes, and gut‐associated lymphoid tissues. To identify genes associated with this defective phenotype in spleen, we applied a gene profiling approach, including subtractive cloning and gene array hybridizations, to mice with combined TNF/LT deficiency. The differentially expressed genes identified by these techniques was then evaluated by Northern blot analysis for splenic expression in knockout mice with single LTα or single TNF deficiency. Most of the genes detected in this analysis are directly or indirectly associated with disrupted LT and not TNF signaling.
ISSN:0741-5400
1938-3673
DOI:10.1189/jlb.68.1.151