Mass spectrometry: A tool for the identification of proteins separated by gels

Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high‐resolution two‐dimensional (2‐D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments dur...

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Veröffentlicht in:Electrophoresis 2000-06, Vol.21 (11), p.2105-2114
Hauptverfasser: Lahm, Hans-Werner, Langen, Hanno
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container_title Electrophoresis
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description Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high‐resolution two‐dimensional (2‐D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.
doi_str_mv 10.1002/1522-2683(20000601)21:11<2105::AID-ELPS2105>3.0.CO;2-M
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subjects Automation
Database Management Systems
Electrophoresis, Gel, Two-Dimensional
Mass spectrometry
Mass Spectrometry - methods
Peptide Mapping
Protein identification
Proteins - chemistry
Proteins - isolation & purification
Proteomics
Review
Two-dimensional gel electrophoresis
title Mass spectrometry: A tool for the identification of proteins separated by gels
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