Mass spectrometry: A tool for the identification of proteins separated by gels

Mass spectrometry: A tool for the identification of proteins separated by gels

Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high‐resolution two‐dimensional (2‐D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments dur...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Electrophoresis 2000-06, Vol.21 (11), p.2105-2114
Hauptverfasser: Lahm, Hans-Werner, Langen, Hanno
Format: Artikel
Sprache:eng
Schlagworte:
Automation
Database Management Systems
Electrophoresis, Gel, Two-Dimensional
Mass spectrometry
Mass Spectrometry - methods
Peptide Mapping
Protein identification
Proteins - chemistry
Proteins - isolation & purification
Proteomics
Review
Two-dimensional gel electrophoresis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high‐resolution two‐dimensional (2‐D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683(20000601)21:11<2105::AID-ELPS2105>3.0.CO;2-M