Sequence and transfection of BoLA-DRB3 cDNAs

Summary Bovine MHC (BoLA‐) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full‐length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA‐DRB3...

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Veröffentlicht in:	Animal genetics 2000-06, Vol.31 (3), p.219-222
Hauptverfasser:	Russell, G C, Fraser, D C, Craigmile, S, Oliver, R A, Dutia, B M, Glass, E J
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Amino Acid Sequence Animals Antibodies, Monoclonal Antibody Specificity cattle Cattle - genetics Cattle - immunology DH22A gene DH24A gene DH8A gene DNA, Complementary - genetics DRB3 gene Evolution, Molecular Genes, MHC Class II Haplotypes histocompatibility antigen HLA Histocompatibility Antigens Class II - genetics HLA-DR Antigens - genetics HLA-DRB3 Chains MHC genes Molecular Sequence Data phenotyping Sequence Analysis, Protein Sequence Homology, Amino Acid Transfection
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Zusammenfassung:	Summary Bovine MHC (BoLA‐) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full‐length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA‐DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.
ISSN:	0268-9146 1365-2052
DOI:	10.1046/j.1365-2052.2000.00630.x