Isolation of Arabidopsis Mutants Lacking Components of Acquired Thermotolerance
Acquired thermotolerance is a complex physiological phenomenon that enables plants to survive normally lethal temperatures. This study characterizes the temperature sensitivity of Arabidopsis using a chlorophyll accumulation bioassay, describes a procedure for selection of acquired thermotolerance m...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2000-06, Vol.123 (2), p.575-587 |
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| creator | Burke, John J. O'Mahony, Patrick J. Oliver, Melvin J. |
| description | Acquired thermotolerance is a complex physiological phenomenon that enables plants to survive normally lethal temperatures. This study characterizes the temperature sensitivity of Arabidopsis using a chlorophyll accumulation bioassay, describes a procedure for selection of acquired thermotolerance mutants, and provides the physiological characterization of one mutant (AtTS02) isolated by this procedure. Exposure of etiolated Arabidopsis seedlings to 48°C or 50°C for 30 min blocks subsequent chlorophyll accumulation and is eventually lethal. Arabidopsis seedlings can be protected against the effects of a 50°C, 30-min challenge by a 4-h pre-incubation at 38°C. By the use of the milder challenge, 44°C for 30 min, and protective pretreatment, mutants lacking components of the acquired thermotolerance system were isolated. Putative mutants isolated by this procedure exhibited chlorophyll accumulation levels (our measure of acquired thermotolerance) ranging from 10% to 98% of control seedling levels following pre-incubation at 38°C and challenge at 50°C. The induction temperatures for maximum acquired thermotolerance prior to a high temperature challenge were the same in AtTS02 and RLD seedlings, although the absolute level of chlorophyll accumulation was reduced in the mutant. Genetic analysis showed that the loss of acquired thermotolerance in AtTS02 was a recessive trait. The pattern of proteins synthesized at 25°C and 38°C in the RLD and AtTS02 revealed the reduction in the level of a 27-kD heat shock protein in AtTS02. Genetic analysis showed that the reduction of this protein level was correlated with the acquired thermotolerance phenotype. |
| doi_str_mv | 10.1104/pp.123.2.575 |
| format | Article |
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This study characterizes the temperature sensitivity of Arabidopsis using a chlorophyll accumulation bioassay, describes a procedure for selection of acquired thermotolerance mutants, and provides the physiological characterization of one mutant (AtTS02) isolated by this procedure. Exposure of etiolated Arabidopsis seedlings to 48°C or 50°C for 30 min blocks subsequent chlorophyll accumulation and is eventually lethal. Arabidopsis seedlings can be protected against the effects of a 50°C, 30-min challenge by a 4-h pre-incubation at 38°C. By the use of the milder challenge, 44°C for 30 min, and protective pretreatment, mutants lacking components of the acquired thermotolerance system were isolated. Putative mutants isolated by this procedure exhibited chlorophyll accumulation levels (our measure of acquired thermotolerance) ranging from 10% to 98% of control seedling levels following pre-incubation at 38°C and challenge at 50°C. The induction temperatures for maximum acquired thermotolerance prior to a high temperature challenge were the same in AtTS02 and RLD seedlings, although the absolute level of chlorophyll accumulation was reduced in the mutant. Genetic analysis showed that the loss of acquired thermotolerance in AtTS02 was a recessive trait. The pattern of proteins synthesized at 25°C and 38°C in the RLD and AtTS02 revealed the reduction in the level of a 27-kD heat shock protein in AtTS02. Genetic analysis showed that the reduction of this protein level was correlated with the acquired thermotolerance phenotype.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.123.2.575</identifier><identifier>PMID: 10859187</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Accumulation ; Agronomy. Soil science and plant productions ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - physiology ; AtTS02 protein ; Bioassay ; Bioassays ; Biological and medical sciences ; Chlorophyll ; Chlorophylls ; Cotyledons ; Economic plant physiology ; Environmental Stress and Adaptation ; Fundamental and applied biological sciences. Psychology ; Growth and development ; Heat shock response ; Heat tolerance ; High temperature ; Hot Temperature ; Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence ; Mutants ; Mutation ; Phenotypes ; Physical agents ; Physiology ; Plant physiology and development ; Plant Proteins - isolation & purification ; Plants ; Seedlings ; Shock heating ; Vegetative apparatus, growth and morphogenesis. 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This study characterizes the temperature sensitivity of Arabidopsis using a chlorophyll accumulation bioassay, describes a procedure for selection of acquired thermotolerance mutants, and provides the physiological characterization of one mutant (AtTS02) isolated by this procedure. Exposure of etiolated Arabidopsis seedlings to 48°C or 50°C for 30 min blocks subsequent chlorophyll accumulation and is eventually lethal. Arabidopsis seedlings can be protected against the effects of a 50°C, 30-min challenge by a 4-h pre-incubation at 38°C. By the use of the milder challenge, 44°C for 30 min, and protective pretreatment, mutants lacking components of the acquired thermotolerance system were isolated. Putative mutants isolated by this procedure exhibited chlorophyll accumulation levels (our measure of acquired thermotolerance) ranging from 10% to 98% of control seedling levels following pre-incubation at 38°C and challenge at 50°C. The induction temperatures for maximum acquired thermotolerance prior to a high temperature challenge were the same in AtTS02 and RLD seedlings, although the absolute level of chlorophyll accumulation was reduced in the mutant. Genetic analysis showed that the loss of acquired thermotolerance in AtTS02 was a recessive trait. The pattern of proteins synthesized at 25°C and 38°C in the RLD and AtTS02 revealed the reduction in the level of a 27-kD heat shock protein in AtTS02. Genetic analysis showed that the reduction of this protein level was correlated with the acquired thermotolerance phenotype.</description><subject>Accumulation</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>AtTS02 protein</subject><subject>Bioassay</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>Chlorophyll</subject><subject>Chlorophylls</subject><subject>Cotyledons</subject><subject>Economic plant physiology</subject><subject>Environmental Stress and Adaptation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth and development</subject><subject>Heat shock response</subject><subject>Heat tolerance</subject><subject>High temperature</subject><subject>Hot Temperature</subject><subject>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Phenotypes</subject><subject>Physical agents</subject><subject>Physiology</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plants</subject><subject>Seedlings</subject><subject>Shock heating</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0U1r3DAQBmARWpJNmluOoZgScupuZ2TJko9hyRdsySU9C1ket97aliPZh_z7atmlDb3kJKF5GND7MnaBsEIE8W0cV8jzFV9JJY_YAmXOl1wK_YEtANIdtC5P2GmMWwDAHMUxO0HQskStFuzpMfrOTq0fMt9kN8FWbe3H2Mbs-zzZYYrZxrrf7fAzW_t-9APtnnbSvcxtoDp7_kWh95PvKNjB0Sf2sbFdpPPDecZ-3N0-rx-Wm6f7x_XNZulEUU7LmkgDybIoNeYVSVsVlbBSaasUSkccrcprQkTb1NDIwlWkS-G0EmhB1fkZu97vHYN_mSlOpm-jo66zA_k5GoUcgGv-LkQlpSwUJPjlP7j1cxjSJwxHXWDKtEjo6x654GMM1JgxtL0NrwbB7Oow42hSHYabVEfinw8756qn-g3e55_A1QHY6GzX7DJs4z8nQOS6TOxyz7Zx8uHvWHBV8rTlD_0jmy8</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Burke, John J.</creator><creator>O'Mahony, Patrick J.</creator><creator>Oliver, Melvin J.</creator><general>American Society of Plant Physiologists</general><general>American Society of Plant Biologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000601</creationdate><title>Isolation of Arabidopsis Mutants Lacking Components of Acquired Thermotolerance</title><author>Burke, John J. ; O'Mahony, Patrick J. ; Oliver, Melvin J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-dee80e5969813be5ab6b4a578a7715ce21a73de111afd0f56cbe894c8741a07d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Accumulation</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>AtTS02 protein</topic><topic>Bioassay</topic><topic>Bioassays</topic><topic>Biological and medical sciences</topic><topic>Chlorophyll</topic><topic>Chlorophylls</topic><topic>Cotyledons</topic><topic>Economic plant physiology</topic><topic>Environmental Stress and Adaptation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth and development</topic><topic>Heat shock response</topic><topic>Heat tolerance</topic><topic>High temperature</topic><topic>Hot Temperature</topic><topic>Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Phenotypes</topic><topic>Physical agents</topic><topic>Physiology</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plants</topic><topic>Seedlings</topic><topic>Shock heating</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burke, John J.</creatorcontrib><creatorcontrib>O'Mahony, Patrick J.</creatorcontrib><creatorcontrib>Oliver, Melvin J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burke, John J.</au><au>O'Mahony, Patrick J.</au><au>Oliver, Melvin J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Arabidopsis Mutants Lacking Components of Acquired Thermotolerance</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>123</volume><issue>2</issue><spage>575</spage><epage>587</epage><pages>575-587</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Acquired thermotolerance is a complex physiological phenomenon that enables plants to survive normally lethal temperatures. This study characterizes the temperature sensitivity of Arabidopsis using a chlorophyll accumulation bioassay, describes a procedure for selection of acquired thermotolerance mutants, and provides the physiological characterization of one mutant (AtTS02) isolated by this procedure. Exposure of etiolated Arabidopsis seedlings to 48°C or 50°C for 30 min blocks subsequent chlorophyll accumulation and is eventually lethal. Arabidopsis seedlings can be protected against the effects of a 50°C, 30-min challenge by a 4-h pre-incubation at 38°C. By the use of the milder challenge, 44°C for 30 min, and protective pretreatment, mutants lacking components of the acquired thermotolerance system were isolated. Putative mutants isolated by this procedure exhibited chlorophyll accumulation levels (our measure of acquired thermotolerance) ranging from 10% to 98% of control seedling levels following pre-incubation at 38°C and challenge at 50°C. The induction temperatures for maximum acquired thermotolerance prior to a high temperature challenge were the same in AtTS02 and RLD seedlings, although the absolute level of chlorophyll accumulation was reduced in the mutant. Genetic analysis showed that the loss of acquired thermotolerance in AtTS02 was a recessive trait. The pattern of proteins synthesized at 25°C and 38°C in the RLD and AtTS02 revealed the reduction in the level of a 27-kD heat shock protein in AtTS02. Genetic analysis showed that the reduction of this protein level was correlated with the acquired thermotolerance phenotype.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>10859187</pmid><doi>10.1104/pp.123.2.575</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Accumulation Agronomy. Soil science and plant productions Arabidopsis Arabidopsis - genetics Arabidopsis - physiology AtTS02 protein Bioassay Bioassays Biological and medical sciences Chlorophyll Chlorophylls Cotyledons Economic plant physiology Environmental Stress and Adaptation Fundamental and applied biological sciences. Psychology Growth and development Heat shock response Heat tolerance High temperature Hot Temperature Morphogenesis, differentiation, rhizogenesis, tuberization. Senescence Mutants Mutation Phenotypes Physical agents Physiology Plant physiology and development Plant Proteins - isolation & purification Plants Seedlings Shock heating Vegetative apparatus, growth and morphogenesis. Senescence |
| title | Isolation of Arabidopsis Mutants Lacking Components of Acquired Thermotolerance |
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