A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding

The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competit...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2000-05, Vol.10 (9), p.951-954
Hauptverfasser:	Connolly, M D, Park, S B, Reedy, B M, Standaert, R F
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - antagonists & inhibitors alpha Karyopherins Amino Acid Sequence Antigens, Polyomavirus Transforming - chemistry Binding, Competitive - drug effects Biotin - chemistry Cell Nucleus - drug effects Cell Nucleus - metabolism Colorimetry Indicators and Reagents Indoles - chemistry Molecular Sequence Data Nuclear Proteins - drug effects Nuclear Proteins - metabolism Peptides - chemical synthesis Peptides - pharmacology Protein Binding Receptors, Cytoplasmic and Nuclear - drug effects Receptors, Cytoplasmic and Nuclear - metabolism Streptavidin
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Beschreibung
Zusammenfassung:	The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
ISSN:	0960-894X
DOI:	10.1016/S0960-894X(00)00134-7