A DNA polymerase ϵ inhibitor activates the ribo and deoxyribo modes of primase expression and induces a unique phenomenon of primer accumulation

A DNA polymerase ϵ inhibitor activates the ribo and deoxyribo modes of primase expression and induces a unique phenomenon of primer accumulation

Carbonyldiphosphonate (COMDP), a selective inhibitor of DNA polymerase (pol) ϵ, strongly stimulates expression of the ribo and deoxyribo modes of primase (Pr) activities of the Pr–DNA pol α enzyme complex associated with special cytoplasmic nucleoprotein complexes of chicken leukemic myeloblasts [J....

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Veröffentlicht in:FEBS letters 2001-09, Vol.505 (1), p.141-146
1. Verfasser: Riman, J
Format: Artikel
Sprache:eng
Schlagworte:
Carbonyldiphosphonate
Deoxyribonucleotides - metabolism
Diphosphonates - pharmacology
DNA Polymerase I - metabolism
DNA Polymerase II - antagonists & inhibitors
DNA polymerase α
DNA Primase - drug effects
DNA Primase - metabolism
DNA Primers - metabolism
Enzyme Inhibitors - pharmacology
Mimosine
Mimosine - metabolism
Primase
Primer molecule
Ribonucleotides - metabolism
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Zusammenfassung:Carbonyldiphosphonate (COMDP), a selective inhibitor of DNA polymerase (pol) ϵ, strongly stimulates expression of the ribo and deoxyribo modes of primase (Pr) activities of the Pr–DNA pol α enzyme complex associated with special cytoplasmic nucleoprotein complexes of chicken leukemic myeloblasts [J. Řı́man and A. Šulová, Acta Virol. 41 (1997) 181–214]. Besides stimulation, COMDP uncouples the Pr activities from those of DNA pol α, inducing in this way a unique phenomenon of accumulation of primers of basic length. In the presence of dNTPs, the COMDP effect is counteracted by excess of mimosine. The mutually exclusive effects of these agents are discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02808-3