Glucocorticoids Regulate Pituitary Growth Hormone Secretagogue Receptor Gene Expression
Glucocorticoids regulate growth hormone (GH) secretion by modulating both hypothalamic and pituitary function. At the level of the pituitary, glucocorticoids increase GH and GH‐releasing hormone receptor (GHRH‐R) gene expression. To test if glucocorticoids might also regulate the pituitary expressio...
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| Veröffentlicht in: | Journal of neuroendocrinology 2000-06, Vol.12 (6), p.481-485 |
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| creator | Tamura, H. Kamegai, J. Sugihara, H. Kineman, R. D. Frohman, L. A. Wakabayashi, I. |
| description | Glucocorticoids regulate growth hormone (GH) secretion by modulating both hypothalamic and pituitary function. At the level of the pituitary, glucocorticoids increase GH and GH‐releasing hormone receptor (GHRH‐R) gene expression. To test if glucocorticoids might also regulate the pituitary expression of the recently identified GH secretagogue (GHS) receptor, GHS‐R; adult male rats were adrenalectomized or sham operated, and treated with the synthetic glucocorticoid (dexamethasone, 200 µg/day) or vehicle for 8 days. Pituitary GHS‐R mRNA levels were assessed by reverse transcriptase polymerase chain reaction (RT‐PCR). Adrenalectomy decreased pituitary GHS‐R mRNA to 45% of vehicle‐treated, sham‐operated rats (P |
| doi_str_mv | 10.1046/j.1365-2826.2000.00446.x |
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D. ; Frohman, L. A. ; Wakabayashi, I.</creator><creatorcontrib>Tamura, H. ; Kamegai, J. ; Sugihara, H. ; Kineman, R. D. ; Frohman, L. A. ; Wakabayashi, I.</creatorcontrib><description>Glucocorticoids regulate growth hormone (GH) secretion by modulating both hypothalamic and pituitary function. At the level of the pituitary, glucocorticoids increase GH and GH‐releasing hormone receptor (GHRH‐R) gene expression. To test if glucocorticoids might also regulate the pituitary expression of the recently identified GH secretagogue (GHS) receptor, GHS‐R; adult male rats were adrenalectomized or sham operated, and treated with the synthetic glucocorticoid (dexamethasone, 200 µg/day) or vehicle for 8 days. Pituitary GHS‐R mRNA levels were assessed by reverse transcriptase polymerase chain reaction (RT‐PCR). Adrenalectomy decreased pituitary GHS‐R mRNA to 45% of vehicle‐treated, sham‐operated rats (P < 0.05). Administration of dexamethasone increased GHS‐R mRNA levels in sham‐operated as well as in adrenalectomized rats (199 ± 24% (P < 0.05) and 369 ± 48% (P < 0.01) of vehicle‐treated controls). Addition of dexamethasone to primary rat pituitary cell cultures increased GHS‐R mRNA levels in a dose‐ and time‐dependent manner while the transcriptional inhibitor, actinomycin D, completely blocked the stimulatory action of dexamethasone. Taken together, these results suggest glucocorticoids directly increase pituitary GHS‐R mRNA levels by stimulating GHS‐R gene transcription.</description><identifier>ISSN: 0953-8194</identifier><identifier>EISSN: 1365-2826</identifier><identifier>DOI: 10.1046/j.1365-2826.2000.00446.x</identifier><identifier>PMID: 10844575</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adrenalectomy ; Animals ; Dactinomycin - pharmacology ; Dexamethasone - pharmacology ; Dose-Response Relationship, Drug ; Gene Expression Regulation - drug effects ; glucocorticoid ; Glucocorticoids - pharmacology ; Growth hormone secretagogue ; growth hormone secretagogue receptor ; Male ; Nucleic Acid Synthesis Inhibitors - pharmacology ; pituitary ; Rats ; Rats, Sprague-Dawley ; Receptors, Neuropeptide - genetics ; Receptors, Pituitary Hormone-Regulating Hormone - genetics ; RNA, Messenger - metabolism ; Time Factors</subject><ispartof>Journal of neuroendocrinology, 2000-06, Vol.12 (6), p.481-485</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Jun 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4956-b83a355d8c99cf8534feed226a6c486de0146463a73dbce6b4dcf9715a5c8e3e3</citedby><cites>FETCH-LOGICAL-c4956-b83a355d8c99cf8534feed226a6c486de0146463a73dbce6b4dcf9715a5c8e3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2826.2000.00446.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2826.2000.00446.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10844575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamura, H.</creatorcontrib><creatorcontrib>Kamegai, J.</creatorcontrib><creatorcontrib>Sugihara, H.</creatorcontrib><creatorcontrib>Kineman, R. D.</creatorcontrib><creatorcontrib>Frohman, L. A.</creatorcontrib><creatorcontrib>Wakabayashi, I.</creatorcontrib><title>Glucocorticoids Regulate Pituitary Growth Hormone Secretagogue Receptor Gene Expression</title><title>Journal of neuroendocrinology</title><addtitle>J Neuroendocrinol</addtitle><description>Glucocorticoids regulate growth hormone (GH) secretion by modulating both hypothalamic and pituitary function. At the level of the pituitary, glucocorticoids increase GH and GH‐releasing hormone receptor (GHRH‐R) gene expression. To test if glucocorticoids might also regulate the pituitary expression of the recently identified GH secretagogue (GHS) receptor, GHS‐R; adult male rats were adrenalectomized or sham operated, and treated with the synthetic glucocorticoid (dexamethasone, 200 µg/day) or vehicle for 8 days. Pituitary GHS‐R mRNA levels were assessed by reverse transcriptase polymerase chain reaction (RT‐PCR). Adrenalectomy decreased pituitary GHS‐R mRNA to 45% of vehicle‐treated, sham‐operated rats (P < 0.05). Administration of dexamethasone increased GHS‐R mRNA levels in sham‐operated as well as in adrenalectomized rats (199 ± 24% (P < 0.05) and 369 ± 48% (P < 0.01) of vehicle‐treated controls). Addition of dexamethasone to primary rat pituitary cell cultures increased GHS‐R mRNA levels in a dose‐ and time‐dependent manner while the transcriptional inhibitor, actinomycin D, completely blocked the stimulatory action of dexamethasone. Taken together, these results suggest glucocorticoids directly increase pituitary GHS‐R mRNA levels by stimulating GHS‐R gene transcription.</description><subject>Adrenalectomy</subject><subject>Animals</subject><subject>Dactinomycin - pharmacology</subject><subject>Dexamethasone - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gene Expression Regulation - drug effects</subject><subject>glucocorticoid</subject><subject>Glucocorticoids - pharmacology</subject><subject>Growth hormone secretagogue</subject><subject>growth hormone secretagogue receptor</subject><subject>Male</subject><subject>Nucleic Acid Synthesis Inhibitors - pharmacology</subject><subject>pituitary</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Neuropeptide - genetics</subject><subject>Receptors, Pituitary Hormone-Regulating Hormone - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><issn>0953-8194</issn><issn>1365-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1rFDEUhoModq3-BRm88G6myeRzwBspu9OWUou2VLwJ2cyZNevsZk0ydPvvzXZKEa-8OgfO874cHoQKgiuCmThZV4QKXtaqFlWNMa4wZkxU-xdo9nx4iWa44bRUpGFH6E2Ma4yJ5BS_RkcEK8a45DN01w6j9daH5Kx3XSy-wmocTILi2qXRJRMeijb4-_SzOPNh47dQfAMbIJmVX42QcQu75EPRQj7N97sAMTq_fYte9WaI8O5pHqPbxfzm9Ky8_NKen36-LC1ruCiXihrKeads09heccp6gK6uhRGWKdEBJkwwQY2k3dKCWLLO9o0k3HCrgAI9Rh-n3l3wv0eISW9ctDAMZgt-jFoSImtK6wx--Adc-zFs82-aNA2TjFKSITVBNvgYA_R6F9wmO9AE64N5vdYHwfogWB_M60fzep-j75_6x-UGur-Ck-oMfJqAezfAw38X64ureV5yvJziLibYP8dN-KWFpJLru6tWL_B39mPRXmtG_wCqdKGb</recordid><startdate>200006</startdate><enddate>200006</enddate><creator>Tamura, H.</creator><creator>Kamegai, J.</creator><creator>Sugihara, H.</creator><creator>Kineman, R. D.</creator><creator>Frohman, L. A.</creator><creator>Wakabayashi, I.</creator><general>Blackwell Science Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200006</creationdate><title>Glucocorticoids Regulate Pituitary Growth Hormone Secretagogue Receptor Gene Expression</title><author>Tamura, H. ; Kamegai, J. ; Sugihara, H. ; Kineman, R. D. ; Frohman, L. A. ; Wakabayashi, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4956-b83a355d8c99cf8534feed226a6c486de0146463a73dbce6b4dcf9715a5c8e3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Adrenalectomy</topic><topic>Animals</topic><topic>Dactinomycin - pharmacology</topic><topic>Dexamethasone - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gene Expression Regulation - drug effects</topic><topic>glucocorticoid</topic><topic>Glucocorticoids - pharmacology</topic><topic>Growth hormone secretagogue</topic><topic>growth hormone secretagogue receptor</topic><topic>Male</topic><topic>Nucleic Acid Synthesis Inhibitors - pharmacology</topic><topic>pituitary</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Neuropeptide - genetics</topic><topic>Receptors, Pituitary Hormone-Regulating Hormone - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamura, H.</creatorcontrib><creatorcontrib>Kamegai, J.</creatorcontrib><creatorcontrib>Sugihara, H.</creatorcontrib><creatorcontrib>Kineman, R. 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A.</creatorcontrib><creatorcontrib>Wakabayashi, I.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroendocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamura, H.</au><au>Kamegai, J.</au><au>Sugihara, H.</au><au>Kineman, R. D.</au><au>Frohman, L. A.</au><au>Wakabayashi, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucocorticoids Regulate Pituitary Growth Hormone Secretagogue Receptor Gene Expression</atitle><jtitle>Journal of neuroendocrinology</jtitle><addtitle>J Neuroendocrinol</addtitle><date>2000-06</date><risdate>2000</risdate><volume>12</volume><issue>6</issue><spage>481</spage><epage>485</epage><pages>481-485</pages><issn>0953-8194</issn><eissn>1365-2826</eissn><abstract>Glucocorticoids regulate growth hormone (GH) secretion by modulating both hypothalamic and pituitary function. At the level of the pituitary, glucocorticoids increase GH and GH‐releasing hormone receptor (GHRH‐R) gene expression. To test if glucocorticoids might also regulate the pituitary expression of the recently identified GH secretagogue (GHS) receptor, GHS‐R; adult male rats were adrenalectomized or sham operated, and treated with the synthetic glucocorticoid (dexamethasone, 200 µg/day) or vehicle for 8 days. Pituitary GHS‐R mRNA levels were assessed by reverse transcriptase polymerase chain reaction (RT‐PCR). Adrenalectomy decreased pituitary GHS‐R mRNA to 45% of vehicle‐treated, sham‐operated rats (P < 0.05). Administration of dexamethasone increased GHS‐R mRNA levels in sham‐operated as well as in adrenalectomized rats (199 ± 24% (P < 0.05) and 369 ± 48% (P < 0.01) of vehicle‐treated controls). Addition of dexamethasone to primary rat pituitary cell cultures increased GHS‐R mRNA levels in a dose‐ and time‐dependent manner while the transcriptional inhibitor, actinomycin D, completely blocked the stimulatory action of dexamethasone. Taken together, these results suggest glucocorticoids directly increase pituitary GHS‐R mRNA levels by stimulating GHS‐R gene transcription.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10844575</pmid><doi>10.1046/j.1365-2826.2000.00446.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Adrenalectomy Animals Dactinomycin - pharmacology Dexamethasone - pharmacology Dose-Response Relationship, Drug Gene Expression Regulation - drug effects glucocorticoid Glucocorticoids - pharmacology Growth hormone secretagogue growth hormone secretagogue receptor Male Nucleic Acid Synthesis Inhibitors - pharmacology pituitary Rats Rats, Sprague-Dawley Receptors, Neuropeptide - genetics Receptors, Pituitary Hormone-Regulating Hormone - genetics RNA, Messenger - metabolism Time Factors |
| title | Glucocorticoids Regulate Pituitary Growth Hormone Secretagogue Receptor Gene Expression |
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