COMPARISON OF NON-VIRAL TRANSFECTION METHODS IN MELANOMA CELL PRIMARY CULTURES

Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58±9%) as compared to lipofection (23±9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 p...

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Veröffentlicht in:	Cytokine (Philadelphia, Pa.) Pa.), 2000-06, Vol.12 (6), p.828-833
Hauptverfasser:	Micka, Bettina, Trojaneck, Beate, Niemitz, Sigrun, Lefterova, Petja, Kruopis, Susanne, Huhn, Dieter, Wittig, Burghardt, Schadendorf, Dirk, Schmidt-Wolf, Ingo G.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD - analysis Cation Exchange Resins Drug Carriers Electroporation - methods Genes, Reporter Green Fluorescent Proteins Humans Immunophenotyping interleukin 2/electroporation/lipofection/melanoma cells Interleukin-2 - analysis Interleukin-2 - genetics Lipids Luminescent Proteins - analysis Luminescent Proteins - genetics Melanoma Recombinant Proteins - analysis Recombinant Proteins - biosynthesis Transfection - methods Tumor Cells, Cultured
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Zusammenfassung:	Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58±9%) as compared to lipofection (23±9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 persisted for up to 3 weeks after electroporation. The increase in sensitivity against immunologic effector cells by transfection with IL-2 was not significant. Our results show the feasibility of a gene tranfer into primary human melanoma cells, different from retroviral transduction.
ISSN:	1043-4666 1096-0023
DOI:	10.1006/cyto.1999.0621