Comparison of Automated Short Tandem Repeat and Manual Variable Number of Tandem Repeat Analysis of Chimerism in Bone Marrow Transplant Patients

Hematopoietic chimerism can be monitored in bone marrow transplant patients at DNA polymorphic sites. In this study, allele detection and quantification by ethidium bromide-stained agarose gels were compared with automated fluorescent sizing on an artificially mixed system and on chimeric post-trans...

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Veröffentlicht in:	Diagnostic molecular pathology 2000-06, Vol.9 (2), p.91-97
Hauptverfasser:	Millson, Alison S, Spangler, Frank L, Wittwer, Carl T, Lyon, Elaine
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Autoanalysis - economics Autoanalysis - methods Bone Marrow Cells Bone Marrow Transplantation DNA - analysis Electrophoresis, Agar Gel - economics Electrophoresis, Agar Gel - methods Humans Minisatellite Repeats - genetics Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods Reproducibility of Results Sensitivity and Specificity Transplantation Chimera - genetics
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