Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius
Abstract The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion...
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| Veröffentlicht in: | Planta medica 2001-08, Vol.67 (6), p.587-589 |
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| container_title | Planta medica |
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| creator | Ha, Wai-Yan Yau, Forrest Chung-Fai But, Paul Pui-Hay Wang, Jun Shaw, Pang-Chui |
| description | Abstract
The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.
Abbreviations
DALP:direct amplified of length polymorphism
STS:sequence-tagged site |
| doi_str_mv | 10.1055/s-2001-16483 |
| format | Article |
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The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.
Abbreviations
DALP:direct amplified of length polymorphism
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The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.
Abbreviations
DALP:direct amplified of length polymorphism
STS:sequence-tagged site</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA, Plant</subject><subject>Drugs, Chinese Herbal</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>General pharmacology</subject><subject>Letter</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Panax - classification</subject><subject>Panax - genetics</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - genetics</subject><subject>Plant Roots - genetics</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Tagged Sites</subject><subject>Species Specificity</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1vEzEQBmALUdG0cOOMfEBcypYZ71d8jFqglSI1BzhbXq-duNq1U8-uRP49DokEBw4jXx6_o3kZe49wi1DXX6gQAFhgUy3LV2yBVSkLEAJfswVAKQqQVXnJroieM6skwBt2iViDlFIs2PbeJ2smvhr3g3fe6MnHwKPjaxu2045v4nAYY9rvPI18FfRwIE_83jtnkw2T15MlvtFB_-JbHyh_4i7FkW9u-cvsw8tsXRz8TG_ZhdMD2Xfn95r9_Pb1x91DsX76_ni3WhemwuVUmLKVndTQN61tTCtkh9i2rWnQmL5bgqirplyKWvfO5skHWmG6pu_Agnboymv26ZS7TzEvp0mNnowdBh1snEm1iNBiU2b4-QRNikTJOrVPftTpoBDUsVhF6lis-lNs5h_OuXM32v4vPjeZwccz0GT04JIOxtM_DrM75tyc2LTzdrTqOc4pt0r_3_obnWWPMg</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>Ha, Wai-Yan</creator><creator>Yau, Forrest Chung-Fai</creator><creator>But, Paul Pui-Hay</creator><creator>Wang, Jun</creator><creator>Shaw, Pang-Chui</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius</title><author>Ha, Wai-Yan ; Yau, Forrest Chung-Fai ; But, Paul Pui-Hay ; Wang, Jun ; Shaw, Pang-Chui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-c379b9a0d67e6c729b11777c61ccdb8025463825adfeadf003e2cb6db0e0af1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA, Plant</topic><topic>Drugs, Chinese Herbal</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>General pharmacology</topic><topic>Letter</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Panax - classification</topic><topic>Panax - genetics</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - genetics</topic><topic>Plant Roots - genetics</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Tagged Sites</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ha, Wai-Yan</creatorcontrib><creatorcontrib>Yau, Forrest Chung-Fai</creatorcontrib><creatorcontrib>But, Paul Pui-Hay</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Shaw, Pang-Chui</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ha, Wai-Yan</au><au>Yau, Forrest Chung-Fai</au><au>But, Paul Pui-Hay</au><au>Wang, Jun</au><au>Shaw, Pang-Chui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>67</volume><issue>6</issue><spage>587</spage><epage>589</epage><pages>587-589</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.
Abbreviations
DALP:direct amplified of length polymorphism
STS:sequence-tagged site</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11509992</pmid><doi>10.1055/s-2001-16483</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0943 |
| ispartof | Planta medica, 2001-08, Vol.67 (6), p.587-589 |
| issn | 0032-0943 1439-0221 |
| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Base Sequence Biological and medical sciences DNA, Plant Drugs, Chinese Herbal Electrophoresis, Polyacrylamide Gel General pharmacology Letter Medical sciences Molecular Sequence Data Nucleic Acid Amplification Techniques Panax - classification Panax - genetics Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - genetics Plant Roots - genetics Polymorphism, Genetic Sequence Tagged Sites Species Specificity |
| title | Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius |
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