Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius

Abstract The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion...

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Veröffentlicht in:	Planta medica 2001-08, Vol.67 (6), p.587-589
Hauptverfasser:	Ha, Wai-Yan, Yau, Forrest Chung-Fai, But, Paul Pui-Hay, Wang, Jun, Shaw, Pang-Chui
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences DNA, Plant Drugs, Chinese Herbal Electrophoresis, Polyacrylamide Gel General pharmacology Letter Medical sciences Molecular Sequence Data Nucleic Acid Amplification Techniques Panax - classification Panax - genetics Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - genetics Plant Roots - genetics Polymorphism, Genetic Sequence Tagged Sites Species Specificity
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Zusammenfassung:	Abstract The method of direct amplification of length polymorphism (DALP) was applied to authenticate PANAX GINSENG and P. QUINQUEFOLIUS. A 636-bp DALP fragment was present in all P. GINSENG but absent in all the P. QUINQUEFOLIUS cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials. Abbreviations DALP:direct amplified of length polymorphism STS:sequence-tagged site
ISSN:	0032-0943 1439-0221
DOI:	10.1055/s-2001-16483