D-fructose-binding proteins in bull seminal plasma: isolation and characterisation

The heparin-binding activity of bull seminal plasma proteins was inhibited by D-fructose, D-glucose, inulin, and glycogen; D-galactose, dextran, and mannan had no effect. While the ejaculated sperm-heparin interaction was not influenced by the presence of saccharides, the heparin-binding activity of...

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Veröffentlicht in:	Folia biologica 2001-01, Vol.47 (4), p.113-119
Hauptverfasser:	Liberda, J, Ryslava, H, Vlasakova, M, Ticha, M. (Karlova Univ., Prague (Czech Republic). Katedra Biochemie), Kraus, M, Jonakova, V
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence AMINO ACID SEQUENCES Animals Blotting, Western BULLS Cattle - metabolism Chromatography, Affinity Chromatography, High Pressure Liquid DEXTRANAS DEXTRANE DEXTRANS Dextrans - pharmacology ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel Epididymis - metabolism FRUCTOSA FRUCTOSE Fructose - metabolism Fructose - pharmacology GALACTOSA GALACTOSE Galactose - pharmacology GLICOGENO GLUCOSA GLUCOSE Glucose - pharmacology GLYCOGEN Glycogen - pharmacology GLYCOGENE HEPARIN Heparin - metabolism HEPARINA HEPARINE HPLC INHIBIDORES DE PROTEINASAS INHIBITEUR DE PROTEINASES INULIN Inulin - pharmacology INULINA INULINE Male MANANOS MANNANE MANNANS Mannans - pharmacology Molecular Sequence Data Protein Binding - drug effects PROTEINAS PROTEINASE INHIBITORS PROTEINE PROTEINS RIBONUCLEASAS RIBONUCLEASE RIBONUCLEASES Ribonucleases - chemistry Ribonucleases - isolation & purification Ribonucleases - metabolism SECUENCIAS DE AMINOACIDOS SEMEN Semen - chemistry Seminal Plasma Proteins - isolation & purification Seminal Plasma Proteins - metabolism Seminal Vesicle Secretory Proteins - chemistry Seminal Vesicle Secretory Proteins - isolation & purification Seminal Vesicle Secretory Proteins - metabolism Sequence Analysis, Protein SEQUENCE D'ACIDES AMINES Spermatozoa - drug effects SPERME TAUREAU Tissue Inhibitor of Metalloproteinase-2 - chemistry Tissue Inhibitor of Metalloproteinase-2 - isolation & purification Tissue Inhibitor of Metalloproteinase-2 - metabolism TORO
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Zusammenfassung:	The heparin-binding activity of bull seminal plasma proteins was inhibited by D-fructose, D-glucose, inulin, and glycogen; D-galactose, dextran, and mannan had no effect. While the ejaculated sperm-heparin interaction was not influenced by the presence of saccharides, the heparin-binding activity of epididymal sperm was inhibited by D-fructose. The results of the binding studies were confirmed by affinity chromatography on immobilized heparin followed by elution with monosaccharides. Proteins adsorbed to a heparin-polyacrylamide column and eluted with D-fructose were analyzed by RP HPLC, SDS electrophoresis, and by determination of the N-terminal amino-acid sequence. RNAase dimer, PDC-109, and metalloproteinase inhibitor (TIMP-2) were identified.
ISSN:	0015-5500