Three-dimensional solid-state NMR spectroscopy is essential for resolution of resonances from in-plane residues in uniformly (15)N-labeled helical membrane proteins in oriented lipid bilayers

Uniformly (15)N-labeled samples of membrane proteins with helices aligned parallel to the membrane surface give two-dimensional PISEMA spectra that are highly overlapped due to limited dispersions of (1)H-(15)N dipolar coupling and (15)N chemical shift frequencies. However, resolution is greatly imp...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of magnetic resonance (1997) 2000-05, Vol.144 (1), p.156-161
Hauptverfasser:	Marassi, F M, Ma, C, Gesell, J J, Opella, S J
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animals Antimicrobial Cationic Peptides HIV-1 Human Immunodeficiency Virus Proteins Lipid Bilayers - chemistry Magainins Magnetic Resonance Spectroscopy - methods Membrane Proteins - chemistry Nitrogen Isotopes Peptides - chemistry Protein Structure, Secondary Viral Regulatory and Accessory Proteins - chemistry Xenopus Proteins
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!