Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay
A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples....
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| Veröffentlicht in: | Asian Pacific journal of allergy and immunology 2001-03, Vol.19 (1), p.43-48 |
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| container_title | Asian Pacific journal of allergy and immunology |
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| creator | Sangsiriwut, K Auewarakul, P Suwanagool, S Wasi, C |
| description | A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts. |
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A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. 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| fulltext | fulltext |
| identifier | ISSN: 0125-877X |
| ispartof | Asian Pacific journal of allergy and immunology, 2001-03, Vol.19 (1), p.43-48 |
| issn | 0125-877X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | CD4 Lymphocyte Count CD4-Positive T-Lymphocytes - immunology DNA, Viral - blood DNA, Viral - immunology Endpoint Determination HIV Infections - blood HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Humans Polymerase Chain Reaction - methods Sensitivity and Specificity Thailand |
| title | Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay |
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