Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay

A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples....

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Veröffentlicht in:	Asian Pacific journal of allergy and immunology 2001-03, Vol.19 (1), p.43-48
Hauptverfasser:	Sangsiriwut, K, Auewarakul, P, Suwanagool, S, Wasi, C
Format:	Artikel
Sprache:	eng
Schlagworte:	CD4 Lymphocyte Count CD4-Positive T-Lymphocytes - immunology DNA, Viral - blood DNA, Viral - immunology Endpoint Determination HIV Infections - blood HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Humans Polymerase Chain Reaction - methods Sensitivity and Specificity Thailand
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Zusammenfassung:	A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.
ISSN:	0125-877X