Development of an automated in-line microfiltration system coupled to an HPLC for the determination of solubility

An automated in-line microfiltration system coupled to an HPLC was developed for the determination of solubility. Filtrations and subsequent solubility determinations are performed using a standard Gilson dilutor and autosampler and dual Rheodyne® valves equipped with a filtration assembly and configured to an HPLC system. The solubility data obtained using the automated in-line microfiltration system are in good agreement with the results obtained using conventional manual preparation techniques. Automating this labor-intensive and often variable portion of the solubility determination provides a reliable means of improving both the consistency and quality of solubility data.