Proteins from fish eggs that protect DNA from acid precipitation and inhibit DNA synthesis

We partially characterized proteins that inhibit DNA acid precipitation from various fish eggs ( Sparus aurata, Dicentrarchus labrax, Mugil cephalus and Zeus faber). The active proteins were purified by acetone fractionation. The activity was found to be heat resistant. Of bivalent cations tested on...

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Veröffentlicht in:	Comparative biochemistry and physiology. Toxicology & pharmacology 2001-08, Vol.129 (4), p.369-376
Hauptverfasser:	Tsamis, Vaggelis, Mamuris, Zissis, Panagiotaki, Panagiota, Kouretas, Demetrios
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Fetoproteins Animals Dicentrarchus labrax DNA - biosynthesis DNA acid precipitation DNA Damage DNA synthesis DNA-Directed DNA Polymerase - isolation & purification DNA-Directed DNA Polymerase - metabolism Eggs Female Fetoprotein Fish egg protein Fish eggs Fishes - physiology Fractional Precipitation Hydrogen-Ion Concentration Mugil cephalus PCR Polymerase Chain Reaction Proteins - chemistry Proteins - isolation & purification Solubility Sparus aurata Temperature Zeus faber
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Beschreibung
Zusammenfassung:	We partially characterized proteins that inhibit DNA acid precipitation from various fish eggs ( Sparus aurata, Dicentrarchus labrax, Mugil cephalus and Zeus faber). The active proteins were purified by acetone fractionation. The activity was found to be heat resistant. Of bivalent cations tested only Co 2+ and Cu 2+ exerted a profound promoting effect in the activity from all fish. The protein fraction from Sparus aurata inhibited DNA synthesis in PCR performed by different DNA polymerases. The possible role of DNA protective proteins in fish egg physiology is discussed.
ISSN:	1532-0456 1878-1659
DOI:	10.1016/S1532-0456(01)00215-0