Reactions of heme peroxidases with peroxynitrite

Reactions of heme peroxidases with peroxynitrite

The interaction of peroxynitrite, produced by ozonation of azide, with two heme peroxidases (horseradish peroxidase and lactoperoxidase) was studied. Enzymes retained full activity after incubation with peroxynitrite at neutral pH. Lactoperoxidase alone was found to catalyze peroxynitrite decomposit...

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Veröffentlicht in:IUBMB life 2000-01, Vol.49 (1), p.11-15
Hauptverfasser: Gebicka, L, Gebicki, J L
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Guaiacol - metabolism
Horseradish Peroxidase - metabolism
Kinetics
Lactoperoxidase - metabolism
Nitrates - metabolism
Oxidoreductases - metabolism
Plants - enzymology
Substrate Specificity
Time Factors
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Zusammenfassung:The interaction of peroxynitrite, produced by ozonation of azide, with two heme peroxidases (horseradish peroxidase and lactoperoxidase) was studied. Enzymes retained full activity after incubation with peroxynitrite at neutral pH. Lactoperoxidase alone was found to catalyze peroxynitrite decomposition, whereas horseradish peroxidase accelerated peroxynitrite decomposition only in the presence of certain substrates. For example, in the presence of guaiacol the catalyzing effect was clear, but in the presence of trolox was only noticeable.
ISSN:1521-6543
1521-6551
DOI:10.1080/152165400306304