Zebrafish pdx1 morphant displays defects in pancreas development and digestive organ chirality, and potentially identifies a multipotent pancreas progenitor cell

The role of pdx1 in zebrafish pancreas development was analyzed in pdx1 morphants. When injected at the one-cell stage, a pdx1 morpholino perturbed pancreas development in a dose-dependent manner (Table 1A, B). When analyzed at 48 h postfertilization (hpf), pdx1 morphants had minimal immunoreactive...

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Veröffentlicht in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2001-07, Vol.30 (3), p.137-140
Hauptverfasser: Yee, Nelson S., Yusuff, Shamila, Pack, Michael
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Sprache:eng
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Zusammenfassung:The role of pdx1 in zebrafish pancreas development was analyzed in pdx1 morphants. When injected at the one-cell stage, a pdx1 morpholino perturbed pancreas development in a dose-dependent manner (Table 1A, B). When analyzed at 48 h postfertilization (hpf), pdx1 morphants had minimal immunoreactive insulin, glucagon, or somatostatin, whereas buffer-injected controls had a solitary prominent islet comprising all three hormone-expressing cells (Fig. 1A-H). pdx1 morphants examined at this stage also had minimal exocrine tissue, based on the expression levels in whole mounts of gata-6, an established endodermal marker (not shown). At 72 hpf, the pancreatic islet of pdx1 morphants was smaller than buffer-injected controls (Fig. 1J, N) and there was minimal exocrine tissue, based on the number of cells expressing gata-6 (Fig. 1K, O) or immunoreactive for carboxypeptidase-A (Fig. 1J, N), a marker of differentiated acinar cells. Tissue sectioning confirmed these findings (Fig. 1L, P) and also showed that the main pancreatic duct formed in pdx1 morphants (not shown). Specificity of these results was confirmed via rescue of the pdx1 morphant pancreatic phenotype by microinjected zebrafish pdx1 mRNA, and the presence of a normal pancreas in 48 hpf hhex and cyclooxygenase2 morphants (not shown).
ISSN:1526-954X
1526-968X
DOI:10.1002/gene.1049