Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices
The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize t...
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| Veröffentlicht in: | International journal of artificial organs 2001-06, Vol.24 (6), p.392-396 |
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| container_title | International journal of artificial organs |
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| creator | Vilei, M T Granato, A Ferraresso, C Neri, D Carraro, P Gerunda, G Muraca, M |
| description | The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed higher rates of diazepam metabolism (2.549+/-0.821 microg/h/million cells vs. 0.474+/-0.079 microg/h/million cells rats, p |
| doi_str_mv | 10.1177/039139880102400609 |
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In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed higher rates of diazepam metabolism (2.549+/-0.821 microg/h/million cells vs. 0.474+/-0.079 microg/h/million cells rats, p<0.005, and vs. 0.704+/-0.171 microg/h/million cells in man, p<0.005) and of bilirubin conjugation (21.60116+/-8.433237 micromoles/l/24 h vs. 6.786809+/-2.983758 in man, p<0.001 and vs. 9.956538+/-1.781016 micromoles/l/24 h in rats, p<0.005). Urea synthesis was similar in pig and in human hepatocytes (150+/-46.3 vs. 144.8+/-21.46 nmoles/h/million cells) and it was lower in rats (84.38+/-35.2; p<0.001 vs. man, p<0.02 vs. pig). High liver specific metabolic activities in cultured pig hepatocytes further support their use as a substitue for human cells in bioartificial liver devices.]]></description><identifier>ISSN: 0391-3988</identifier><identifier>EISSN: 1724-6040</identifier><identifier>DOI: 10.1177/039139880102400609</identifier><identifier>PMID: 11482505</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Bilirubin - metabolism ; Cells, Cultured ; Diazepam - metabolism ; Humans ; Liver - cytology ; Liver - metabolism ; Liver, Artificial ; Male ; Rats ; Statistics, Nonparametric ; Swine ; Urea - metabolism</subject><ispartof>International journal of artificial organs, 2001-06, Vol.24 (6), p.392-396</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-f0817d21e028678fd0e6aa64895c704295426bf0d5addc9d5cb06418a3716ec43</citedby><cites>FETCH-LOGICAL-c299t-f0817d21e028678fd0e6aa64895c704295426bf0d5addc9d5cb06418a3716ec43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11482505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vilei, M T</creatorcontrib><creatorcontrib>Granato, A</creatorcontrib><creatorcontrib>Ferraresso, C</creatorcontrib><creatorcontrib>Neri, D</creatorcontrib><creatorcontrib>Carraro, P</creatorcontrib><creatorcontrib>Gerunda, G</creatorcontrib><creatorcontrib>Muraca, M</creatorcontrib><title>Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices</title><title>International journal of artificial organs</title><addtitle>Int J Artif Organs</addtitle><description><![CDATA[The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed higher rates of diazepam metabolism (2.549+/-0.821 microg/h/million cells vs. 0.474+/-0.079 microg/h/million cells rats, p<0.005, and vs. 0.704+/-0.171 microg/h/million cells in man, p<0.005) and of bilirubin conjugation (21.60116+/-8.433237 micromoles/l/24 h vs. 6.786809+/-2.983758 in man, p<0.001 and vs. 9.956538+/-1.781016 micromoles/l/24 h in rats, p<0.005). Urea synthesis was similar in pig and in human hepatocytes (150+/-46.3 vs. 144.8+/-21.46 nmoles/h/million cells) and it was lower in rats (84.38+/-35.2; p<0.001 vs. man, p<0.02 vs. pig). High liver specific metabolic activities in cultured pig hepatocytes further support their use as a substitue for human cells in bioartificial liver devices.]]></description><subject>Animals</subject><subject>Bilirubin - metabolism</subject><subject>Cells, Cultured</subject><subject>Diazepam - metabolism</subject><subject>Humans</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver, Artificial</subject><subject>Male</subject><subject>Rats</subject><subject>Statistics, Nonparametric</subject><subject>Swine</subject><subject>Urea - metabolism</subject><issn>0391-3988</issn><issn>1724-6040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0b-O1DAQBnALgbjl4AUokCsqAmPHsZMSrY4_0kk0UEeOPeaMkjh47JP2hXhONtqVKJBGmil-8zUfY68FvBfCmA_QDqId-h4ESAWgYXjCDsJI1WhQ8JQddtDs4oa9IPoFILRS3XN2I4TqZQfdgf05pmWzOVJaeQp8iz_f8Ye62JXb1fNsC3_AzZbkTgWJ2_NwSjU73PUcHzFz2tDFEB1fsNgpzecr1NWVmFbiceWuzqVm5HSiggs1TVy2M7IXEFLmlXCHU0w2lz0q2vka7vExOqSX7FmwM-Gr675lPz7dfT9-ae6_ff56_HjfODkMpQnQC-OlQJC9Nn3wgNparfqhcwaUHDol9RTAd9Z7N_jOTaCV6G1rhEan2lv29pK75fS7IpVxieRwnu2KqdJoBHQKzA7lBbqciDKGcctxsfk0Chj3dsb_2zk_vbmm12lB_-_lWkf7F_vajWg</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Vilei, M T</creator><creator>Granato, A</creator><creator>Ferraresso, C</creator><creator>Neri, D</creator><creator>Carraro, P</creator><creator>Gerunda, G</creator><creator>Muraca, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010601</creationdate><title>Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices</title><author>Vilei, M T ; Granato, A ; Ferraresso, C ; Neri, D ; Carraro, P ; Gerunda, G ; Muraca, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-f0817d21e028678fd0e6aa64895c704295426bf0d5addc9d5cb06418a3716ec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Bilirubin - metabolism</topic><topic>Cells, Cultured</topic><topic>Diazepam - metabolism</topic><topic>Humans</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver, Artificial</topic><topic>Male</topic><topic>Rats</topic><topic>Statistics, Nonparametric</topic><topic>Swine</topic><topic>Urea - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilei, M T</creatorcontrib><creatorcontrib>Granato, A</creatorcontrib><creatorcontrib>Ferraresso, C</creatorcontrib><creatorcontrib>Neri, D</creatorcontrib><creatorcontrib>Carraro, P</creatorcontrib><creatorcontrib>Gerunda, G</creatorcontrib><creatorcontrib>Muraca, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilei, M T</au><au>Granato, A</au><au>Ferraresso, C</au><au>Neri, D</au><au>Carraro, P</au><au>Gerunda, G</au><au>Muraca, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices</atitle><jtitle>International journal of artificial organs</jtitle><addtitle>Int J Artif Organs</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>24</volume><issue>6</issue><spage>392</spage><epage>396</epage><pages>392-396</pages><issn>0391-3988</issn><eissn>1724-6040</eissn><abstract><![CDATA[The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed higher rates of diazepam metabolism (2.549+/-0.821 microg/h/million cells vs. 0.474+/-0.079 microg/h/million cells rats, p<0.005, and vs. 0.704+/-0.171 microg/h/million cells in man, p<0.005) and of bilirubin conjugation (21.60116+/-8.433237 micromoles/l/24 h vs. 6.786809+/-2.983758 in man, p<0.001 and vs. 9.956538+/-1.781016 micromoles/l/24 h in rats, p<0.005). Urea synthesis was similar in pig and in human hepatocytes (150+/-46.3 vs. 144.8+/-21.46 nmoles/h/million cells) and it was lower in rats (84.38+/-35.2; p<0.001 vs. man, p<0.02 vs. pig). High liver specific metabolic activities in cultured pig hepatocytes further support their use as a substitue for human cells in bioartificial liver devices.]]></abstract><cop>United States</cop><pmid>11482505</pmid><doi>10.1177/039139880102400609</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0391-3988 |
| ispartof | International journal of artificial organs, 2001-06, Vol.24 (6), p.392-396 |
| issn | 0391-3988 1724-6040 |
| language | eng |
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| source | MEDLINE; SAGE Backfile Access |
| subjects | Animals Bilirubin - metabolism Cells, Cultured Diazepam - metabolism Humans Liver - cytology Liver - metabolism Liver, Artificial Male Rats Statistics, Nonparametric Swine Urea - metabolism |
| title | Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices |
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