Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices

Comparison of pig, human and rat hepatocytes as a source of liver specific metabolic functions in culture systems--implications for use in bioartificial liver devices

The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize t...

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Veröffentlicht in:International journal of artificial organs 2001-06, Vol.24 (6), p.392-396
Hauptverfasser: Vilei, M T, Granato, A, Ferraresso, C, Neri, D, Carraro, P, Gerunda, G, Muraca, M
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bilirubin - metabolism
Cells, Cultured
Diazepam - metabolism
Humans
Liver - cytology
Liver - metabolism
Liver, Artificial
Male
Rats
Statistics, Nonparametric
Swine
Urea - metabolism
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Zusammenfassung:The limited availability of human hepatocytes results in the use of animal cells in most bioartificial liver support devices. In the present work, clinically relevant liver specific metabolic activities were compared in rat, pig and human hepatocytes cultured on liver-derived biomatrix to optimize the expression of differentiated functions. Pig hepatocytes showed higher rates of diazepam metabolism (2.549+/-0.821 microg/h/million cells vs. 0.474+/-0.079 microg/h/million cells rats, p
ISSN:0391-3988
1724-6040
DOI:10.1177/039139880102400609