The effect of ex vivo refrigerated storage and cell preservation solution (Cyto-Chex II™) on CD11b expression and oxidative burst activity of dog neutrophils

The expression of CD11b and oxidative burst activity of dog neutrophils undergoing ex vivo refrigerated storage was studied using flow-cytometry. Additionally, the effect of a proprietary cell stabilization reagent (Cyto-Chex™) on the expression of CD11b and oxidative burst activity was studied. Exp...

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Veröffentlicht in:	Veterinary immunology and immunopathology 2000-04, Vol.74 (1), p.59-69
Hauptverfasser:	Ruaux, Craig G, Williams, David A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CD11b CD11b antigen Cell preservation Dog Dogs - immunology Female Flow Cytometry Gene Expression Regulation Integrins Leukocyte antigens Macrophage-1 Antigen - biosynthesis Macrophage-1 Antigen - genetics Macrophage-1 Antigen - immunology Male Neutrophil Neutrophils - chemistry Neutrophils - immunology Oxidative burst Preservation, Biological - methods Preservation, Biological - veterinary Refrigeration Respiratory Burst - immunology Time Factors
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Zusammenfassung:	The expression of CD11b and oxidative burst activity of dog neutrophils undergoing ex vivo refrigerated storage was studied using flow-cytometry. Additionally, the effect of a proprietary cell stabilization reagent (Cyto-Chex™) on the expression of CD11b and oxidative burst activity was studied. Expression of CD11b was very high (>90% positive) on dog neutrophils isolated from peripheral blood. Dog neutrophils showed a rapid and sustained increase in CD11b antigen density ( P
ISSN:	0165-2427 1873-2534
DOI:	10.1016/S0165-2427(00)00159-8