Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pH‐sensitive liposomes

An oligonucleotide of 20 bases, complementary to a region of the sodium/myo‐inositol cotransporter (SMIT) mRNA, was used to investigate the uptake efficiency and activity of transferred antisense oligonucleotides with regard to substrate uptake. We compared the efficiency of oligonucleotide delivery...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:European journal of biochemistry 2000-04, Vol.267 (8), p.2432-2438
Hauptverfasser: Lubrich, Beate, van Calker, Dietrich, Peschka‐Süss, Regine
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:An oligonucleotide of 20 bases, complementary to a region of the sodium/myo‐inositol cotransporter (SMIT) mRNA, was used to investigate the uptake efficiency and activity of transferred antisense oligonucleotides with regard to substrate uptake. We compared the efficiency of oligonucleotide delivery after application of either free or liposome‐encapsulated material. Delivery of liposome‐encapsulated material (marker or oligonucleotides) into astrocytoma cells and primary astrocyte cultures was more effective with pH‐sensitive liposomes [dioleoylphosphatidylethanolamine (DOPE)/cholesteryl hemisuccinate (CHEMS)] than with non‐pH‐sensitive liposomes (soy lecithin) or free material in solution. Antisense activity was evaluated by determination of myo‐inositol uptake and detection of SMIT transcripts by RT‐PCR. Encapsulation of oligonucleotides in pH‐sensitive liposomes increased the inhibition of inositol uptake at least 50‐fold compared with application of free oligonucleotides in solution.
ISSN:0014-2956
1432-1033
DOI:10.1046/j.1432-1327.2000.01255.x