Preparation of oligonucleotides without aldehyde abasic sites

High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2001-07, Vol.11 (14), p.1863-1867
Hauptverfasser:	KROTZ, Achim H, GAUS, Hans, RAVIKUMAR, Vasulinga T, COLE, Douglas L
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehydes - chemistry Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Hydroxylamine - chemistry Nucleic acids Nucleic bases, nucleotides Oligonucleotides - chemistry Oligonucleotides - isolation & purification Purines - chemistry Sequence Deletion - physiology Spectrometry, Mass, Electrospray Ionization Surface-Active Agents - chemistry Water - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites leading to significantly increased retention.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/S0960-894X(01)00307-9