Identification of Human CPI-17, an Inhibitory Phosphoprotein for Myosin Phosphatase

CPI-17 is a phosphorylation-dependent inhibitor of myosin phosphatase. cDNA clones encoding CPI-17 were isolated from a human aorta library. Overlapping clones indicated two isoforms: CPI-17α was 147 residues and mass of 16.7 kDa; CPI-17β (120 residues, mass 13.5 kDa) resulted from a deletion in the...

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Veröffentlicht in:Biochemical and biophysical research communications 2001-07, Vol.285 (4), p.1040-1045
Hauptverfasser: Yamawaki, Koji, Ito, Masaaki, Machida, Hirofumi, Moriki, Nobuyuki, Okamoto, Ryuji, Isaka, Naoki, Shimpo, Hideto, Kohda, Atsushi, Okumura, Katsuzumi, Hartshorne, David J., Nakano, Takeshi
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Sprache:eng
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Zusammenfassung:CPI-17 is a phosphorylation-dependent inhibitor of myosin phosphatase. cDNA clones encoding CPI-17 were isolated from a human aorta library. Overlapping clones indicated two isoforms: CPI-17α was 147 residues and mass of 16.7 kDa; CPI-17β (120 residues, mass 13.5 kDa) resulted from a deletion in the α-isoform of 27 residues, sequence 68–94. N-terminal 67 residues of all CPI-17 isoforms (human, porcine, rat and mouse) were highly conserved (for the human and porcine isoforms the identity was 91%). The presence of the two human isoforms was detected from cDNA sequences amplified by RT-PCR and by Western blots on human aorta. The cloned human CPI-17 gene indicated 4 coding exons and CPI-17β was an alternative splice variant due to deletion of the second exon. FISH analysis located the human CPI-17 gene on chromosome 19q13.1.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5290