25-Hydroxyvitamin D3, the Prohormone of 1,25-Dihydroxyvitamin D3, Inhibits the Proliferation of Primary Prostatic Epithelial Cells

The hormonal metabolite of vitamin D, 1α,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] is known to inhibit the proliferation of prostatic epithelial cells. This has stimulated interest in vitamin D compounds as therapeutic agents for prostate cancer. However, the therapeutic use of 1,25(OH) 2 D 3 is limited because elevations in serum 1,25(OH) 2 D 3 can cause dangerous elevations in serum calcium levels. We wondered whether the prohormone of 1,25(OH) 2 D 3 , 25-hydroxyvitamin D 3 (25-OH-D 3 ), which is much less calcemic, could also achieve antiproliferative effects in prostatic cells. 25-OH-D 3 is converted to 1,25(OH) 2 D 3 by the mitochondrial enzyme 1-α-hydroxylase. We have recently shown that human prostatic cells also possess significant 1-α-hydroxylase activity (Schwartz et al. , Cancer Epidemiol. Biomark. Prev., 7: 391–395, 1998). We studied 1-α-hydroxylase gene expression in four strains of primary human prostatic epithelial cells by reverse transcription PCR amplification (RT-PCR) of 1-α-hydroxylase. Human prostatic stromal cells were negative for 1-α-hydroxylase by RT-PCR. This led us to hypothesize that 25-OH-D 3 would inhibit the proliferation of prostatic epithelial cells because 25-OH-D 3 would be converted to 1,25(OH) 2 D 3 intracellularly. We studied the effects of 25-OH-D 3 and 1,25(OH) 2 D 3 on the proliferation of prostatic epithelial cells using high density growth and clonal growth assays on two different primary cell strains derived from normal human prostatic peripheral zone. 25-OH-D 3 and 1,25(OH) 2 D 3 each inhibited growth in a dose- and time-dependent manner. Growth inhibition was evident at 1 n m , and maximal inhibition was observed at 100 n m within 10–12 days of exposure. The potencies of 25-OH-D 3 and 1,25(OH) 2 D 3 were not significantly different. These data demonstrate that 25-OH-D 3 , which previously was thought to have little biological activity, can become a potent antiproliferative hormone for prostatic cells that express 1-α-hydroxylase. Because 25-OH-D 3 exhibits similar potency to 1,25(OH) 2 D 3 but is less calcemic, 25-OH-D 3 may offer a safer option than 1,25(OH) 2 D 3 for prostate cancer therapy. Moreover, because 25-OH-D 3 is produced endogenously from vitamin D, these findings support a potential role for vitamin D in the chemoprevention of prostate cancer.