Quick sex determination of mouse fetuses

We designed a rapid, simple and accurate PCR method to determine sexual identity of mouse fetuses collected on embryonic day 15. A multiplex PCR amplification was used to detect male-specific sequence (Sry) in DNA extracted from fetal livers through SDS denaturation followed by high salt extraction...

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Veröffentlicht in:	Journal of neuroscience methods 2000-02, Vol.95 (2), p.127-132
Hauptverfasser:	Lambert, Jean-François, Benoit, Brian O, Colvin, Gerald A, Carlson, Jane, Delville, Yvon, Quesenberry, Peter J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Culture Techniques DNA - isolation & purification DNA extraction Female Fetus FISH Fundamental and applied biological sciences. Psychology General aspects. Models. Methods In Situ Hybridization Male Mice Mice, Inbred BALB C Neostriatum - cytology Neural stem cells PCR Polymerase Chain Reaction - methods Rapid Sex Determination Analysis Sexual differentiation Sry Stem Cells - cytology Time Factors Vertebrates: nervous system and sense organs
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Beschreibung
Zusammenfassung:	We designed a rapid, simple and accurate PCR method to determine sexual identity of mouse fetuses collected on embryonic day 15. A multiplex PCR amplification was used to detect male-specific sequence (Sry) in DNA extracted from fetal livers through SDS denaturation followed by high salt extraction and precipitation. This extraction method resulted in sufficiently purified DNA in
ISSN:	0165-0270 1872-678X
DOI:	10.1016/S0165-0270(99)00157-0