A new method for direct introduction of chemicals into a single sieve tube of intact rice [Oryza sativa] plants

We present a novel method for direct introduction of chemicals into a sieve tube of intact rice plants–namely, the method of micro-introduction using stylet of insect (the MUSI method). Fluorescent dyes were successfully introduced into a sieve tube through a severed stylet of planthopper and the di...

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Veröffentlicht in:	Plant and cell physiology 2000-01, Vol.41 (1), p.124-128
Hauptverfasser:	Fujimaki, S. (Tokyo Univ. (Japan)), Fujiwara, T, Hayashi, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Fluorescein-5-isothiocyanate - administration & dosage Fluorescein-5-isothiocyanate - pharmacokinetics Fluorescent Dyes - administration & dosage Fluorescent Dyes - pharmacokinetics INJECTION Insect stylectomy Insecta INYECCION METHODE METHODS METODOS Microinjection Microinjections - methods Microintroduction Microscopy, Fluorescence Oryza - metabolism ORYZA SATIVA Plant Leaves - metabolism PLANT TISSUES Rhodamines - administration & dosage Rhodamines - pharmacokinetics Rice (Oryza sativa L.) Sieve tube TEJIDOS VEGETALES TISSU VEGETAL
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Zusammenfassung:	We present a novel method for direct introduction of chemicals into a sieve tube of intact rice plants–namely, the method of micro-introduction using stylet of insect (the MUSI method). Fluorescent dyes were successfully introduced into a sieve tube through a severed stylet of planthopper and the distribution of the dyes was observed.
ISSN:	0032-0781 1471-9053
DOI:	10.1093/pcp/41.1.124