Gravitational field-flow fractionation for the characterisation of active dry wine yeast

Gravitational field-flow fractionation (GrFFF) is applied to the fractionation of active dry wine yeast. An experimental approach to the analysis of the effects that field variation by changing mobile phase composition and flow-rate have on the fractionation process of standard particles (polystyren...

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Veröffentlicht in:Journal of Chromatography A 2001-06, Vol.919 (2), p.339-347
Hauptverfasser: Sanz, R, Puignou, L, Reschiglian, P, Galceran, M.T
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Sprache:eng
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Zusammenfassung:Gravitational field-flow fractionation (GrFFF) is applied to the fractionation of active dry wine yeast. An experimental approach to the analysis of the effects that field variation by changing mobile phase composition and flow-rate have on the fractionation process of standard particles (polystyrene) was first developed to further obtain effective fractionation of wine yeast by GrFFF. Scanning electron microscopy and Coulter counter particle size measurements were used to monitor the fractionation extent and capabilities of GrFFF to describe the distribution of yeast cells populations.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)00807-X