FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT
Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram....
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| Veröffentlicht in: | Journal of toxicological sciences 2000/02/28, Vol.25(1), pp.41-48 |
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| container_title | Journal of toxicological sciences |
| container_volume | 25 |
| creator | YAMAMOTO, Toshinobu YONEYAMA, Mitsuru IMANISHI, Masanori TAKEUCHI, Masaki |
| description | Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P |
| doi_str_mv | 10.2131/jts.25.41 |
| format | Article |
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When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P<0.01), or nitrobenzene(r=0.80, P<0.01). The results indicated the utility of the light scatter-histogram in flow cytometry as a simple and convenient procedure for the detection of tailless sperm induced by chemical compounds.</description><identifier>ISSN: 0388-1350</identifier><identifier>EISSN: 1880-3989</identifier><identifier>DOI: 10.2131/jts.25.41</identifier><identifier>PMID: 10736789</identifier><identifier>CODEN: JTSCDR</identifier><language>eng</language><publisher>Tokyo: The Japanese Society of Toxicology</publisher><subject>Animals ; Biological and medical sciences ; Flow Cytometry ; General aspects. 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When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P<0.01), or nitrobenzene(r=0.80, P<0.01). The results indicated the utility of the light scatter-histogram in flow cytometry as a simple and convenient procedure for the detection of tailless sperm induced by chemical compounds.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Flow Cytometry</subject><subject>General aspects. Methods</subject><subject>Light</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Scattering, Radiation</subject><subject>Sonication</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Toxicology</subject><issn>0388-1350</issn><issn>1880-3989</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0EFv2yAUB3A0bVrTbod9gQlp1aQdnIHBAY6uQ1pLTjzFrqacEAa8JXKSDpJDv_3sOeqmHQCJ9-M99AfgA0bTGBP8dXcK0ziZUvwKTDDnKCKCi9dgggjnESYJugLXIewQihlK6FtwhREjM8bFBOhFUX6H2aYul7Je5xmcy1pmdV6uYLqa9ystNlVewXIB6zQvCllVsPom10uYpY-VnMO7DazKVZ6lf96Ua5jC7EEu-4sCpvdyVb8Db1rdBff-ct6Ax4Wss4eoKO8HFRlKOY40tsKZVhNKOMJWa8acs5o7QW1jLTKGIBaLxgmWGGcS0WBtGmetNUnrEkRuwOex75M__jq7cFL7bTCu6_TBHc9BMSQEnqEBfvoP7o5nf-j_pjCdcc5ELFivvozK-GMI3rXqyW_32j8rjNSQuupTV3GiKO7tx0vHc7N39h85xtyD2wvQweiu9fpgtuGvIyJBbGB3I9uFk_7hXuran7amc8NELNhsmIrHjeKXovmpvXIH8hsUt5l6</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>YAMAMOTO, Toshinobu</creator><creator>YONEYAMA, Mitsuru</creator><creator>IMANISHI, Masanori</creator><creator>TAKEUCHI, Masaki</creator><general>The Japanese Society of Toxicology</general><general>Japanese Society of Toxicology</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT</title><author>YAMAMOTO, Toshinobu ; YONEYAMA, Mitsuru ; IMANISHI, Masanori ; TAKEUCHI, Masaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4481-a1d9ecfa343801daa77eeda8e94dbdd0cc30729be975cec59b1acbedddc5fe503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Flow Cytometry</topic><topic>General aspects. Methods</topic><topic>Light</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Scattering, Radiation</topic><topic>Sonication</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Toxicology</topic><toplevel>online_resources</toplevel><creatorcontrib>YAMAMOTO, Toshinobu</creatorcontrib><creatorcontrib>YONEYAMA, Mitsuru</creatorcontrib><creatorcontrib>IMANISHI, Masanori</creatorcontrib><creatorcontrib>TAKEUCHI, Masaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of toxicological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMAMOTO, Toshinobu</au><au>YONEYAMA, Mitsuru</au><au>IMANISHI, Masanori</au><au>TAKEUCHI, Masaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT</atitle><jtitle>Journal of toxicological sciences</jtitle><addtitle>J Toxicol Sci</addtitle><date>2000</date><risdate>2000</risdate><volume>25</volume><issue>1</issue><spage>41</spage><epage>48</epage><pages>41-48</pages><issn>0388-1350</issn><eissn>1880-3989</eissn><coden>JTSCDR</coden><abstract>Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P<0.01), or nitrobenzene(r=0.80, P<0.01). The results indicated the utility of the light scatter-histogram in flow cytometry as a simple and convenient procedure for the detection of tailless sperm induced by chemical compounds.</abstract><cop>Tokyo</cop><pub>The Japanese Society of Toxicology</pub><pmid>10736789</pmid><doi>10.2131/jts.25.41</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0388-1350 |
| ispartof | The Journal of Toxicological Sciences, 2000/02/28, Vol.25(1), pp.41-48 |
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| language | eng |
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| subjects | Animals Biological and medical sciences Flow Cytometry General aspects. Methods Light Male Medical sciences Rats Rats, Sprague-Dawley Scattering, Radiation Sonication Spermatozoa - cytology Spermatozoa - drug effects Toxicology |
| title | FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT |
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