FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT

FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT

Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram....

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Veröffentlicht in:Journal of toxicological sciences 2000/02/28, Vol.25(1), pp.41-48
Hauptverfasser: YAMAMOTO, Toshinobu, YONEYAMA, Mitsuru, IMANISHI, Masanori, TAKEUCHI, Masaki
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Flow Cytometry
General aspects. Methods
Light
Male
Medical sciences
Rats
Rats, Sprague-Dawley
Scattering, Radiation
Sonication
Spermatozoa - cytology
Spermatozoa - drug effects
Toxicology
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Zusammenfassung:Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P
ISSN:0388-1350
1880-3989
DOI:10.2131/jts.25.41