Role of glutathione in regulation of hydroperoxidase I in growing Escherichia coli

To examine role of glutathione in regulation of catalases in growing Escherichia coli, katG:: lacZ and katE:: lacZ fusions were transformed into a glutathione-deficient Escherichia coli strain and wild-type parent. In the absence of H 2O 2 and in the presence of the low H 2O 2 concentrations (0.1–3...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Free radical biology & medicine 2001-07, Vol.31 (2), p.250-255
Hauptverfasser: Oktyabrsky, Oleg N, Smirnovam, Galina V, Muzyka, Nadegda G
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:To examine role of glutathione in regulation of catalases in growing Escherichia coli, katG:: lacZ and katE:: lacZ fusions were transformed into a glutathione-deficient Escherichia coli strain and wild-type parent. In the absence of H 2O 2 and in the presence of the low H 2O 2 concentrations (0.1–3 mM), the gshA mutation stimulated katG:: lacZ expression and the total catalase activity in exponential phase. In the absence of H 2O 2, the mutation in gshA also stimulated katE:: lacZ expression. At higher H 2O 2 concentrations, the gshA mutation suppressed katG:: lacZ expression and catalase activity. In stationary and mid-exponential phases, the intracellular concentrations of H 2O 2 in the gshA mutant were markedly increased compared to those in the wild type. These results suggest that glutathione may be involved in regulation of catalases.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(01)00572-X