Role of glutathione in regulation of hydroperoxidase I in growing Escherichia coli
To examine role of glutathione in regulation of catalases in growing Escherichia coli, katG:: lacZ and katE:: lacZ fusions were transformed into a glutathione-deficient Escherichia coli strain and wild-type parent. In the absence of H 2O 2 and in the presence of the low H 2O 2 concentrations (0.1–3...
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Veröffentlicht in: | Free radical biology & medicine 2001-07, Vol.31 (2), p.250-255 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | To examine role of glutathione in regulation of catalases in growing
Escherichia coli,
katG::
lacZ and
katE::
lacZ fusions were transformed into a glutathione-deficient
Escherichia coli strain and wild-type parent. In the absence of H
2O
2 and in the presence of the low H
2O
2 concentrations (0.1–3 mM), the
gshA mutation stimulated
katG::
lacZ expression and the total catalase activity in exponential phase. In the absence of H
2O
2, the mutation in
gshA also stimulated
katE::
lacZ expression. At higher H
2O
2 concentrations, the
gshA mutation suppressed
katG::
lacZ expression and catalase activity. In stationary and mid-exponential phases, the intracellular concentrations of H
2O
2 in the
gshA mutant were markedly increased compared to those in the wild type. These results suggest that glutathione may be involved in regulation of catalases. |
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ISSN: | 0891-5849 1873-4596 |
DOI: | 10.1016/S0891-5849(01)00572-X |