Dialysis cells for controlled DNA: drug binding studies

Dialysis cells for controlled DNA: drug binding studies

An efficient small volume accurate dialysis system has been designed, built and tested for proflavine binding to DNA based upon the side by side design used in the Franz diffusion cell. In a typical experiment 3 cm3 of DNA solution is added to one side and 3 cm3 of ligand to the other with a dialysi...

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Veröffentlicht in:Analyst (London) 2001-01, Vol.126 (6), p.852-854
Hauptverfasser: SANDERS, Karen J, WILES, Harry, RODGER, Alison
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Anti-Infective Agents, Local - metabolism
Biological and medical sciences
Cattle
Dialysis
Diverse techniques
DNA - metabolism
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
proflavine
Proflavine - metabolism
Protein Binding
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Beschreibung
Zusammenfassung:An efficient small volume accurate dialysis system has been designed, built and tested for proflavine binding to DNA based upon the side by side design used in the Franz diffusion cell. In a typical experiment 3 cm3 of DNA solution is added to one side and 3 cm3 of ligand to the other with a dialysis membrane between the two sides of 1 cm in diameter, thereby minimizing the area of dialysis membrane that the solutions are in contact with.
ISSN:0003-2654
1364-5528
DOI:10.1039/b005972m