Detection of Protein Oxidation in Rat-1 Fibroblasts by Fluorescently Labeled Tyramine

Oxidative damage to proteins has been postulated as a major cause of various degenerative diseases including the loss of functional capacity during aging. A prominent target for oxidation by reactive oxygen species (ROS) is the tyrosine residue. Here we present a highly sensitive method for the dete...

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Veröffentlicht in:	Biochemistry (Easton) 2001-07, Vol.40 (26), p.7783-7788
Hauptverfasser:	van der Vlies, Dennis, Wirtz, Karel W. A, Pap, Eward H. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Animals Cell Line Cell Membrane Permeability Cross-Linking Reagents - metabolism Fibroblasts - metabolism Fluorescein - metabolism Fluoresceins - metabolism Fluorescent Dyes - metabolism Free Radicals - metabolism Microscopy, Fluorescence Oxidation-Reduction Proteins - metabolism Rats Reactive Oxygen Species - metabolism Tyramine - metabolism Tyrosine - metabolism
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