A novel in vitro model system to grow films of oral bacteria for the study of human tooth root surface caries
Aims: To develop a simple and flexible novel in vitro model system to grow films of oral bacteria that could be used to study aspects of dental caries. Methods and Results: Standardized suspensions of bacteria were inoculated into Ultrafree‐CL (Millipore) ultrafiltration units at various densities....
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Veröffentlicht in: | Journal of applied microbiology 2001-07, Vol.91 (1), p.139-146 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Aims: To develop a simple and flexible novel in vitro model system to grow films of oral bacteria that could be used to study aspects of dental caries.
Methods and Results: Standardized suspensions of bacteria were inoculated into Ultrafree‐CL (Millipore) ultrafiltration units at various densities. These were incubated for varying time intervals with a range of carbon sources. The bacterial films reproducibly achieved between 107 and 108 cfu cm−2, irrespective of the number inoculated and with no significant changes for 14 d. However, Streptococcus mutans grew through membranes with pores of diameter greater than 0·1 μm after 6 d. Culture of films in sucrose or water for 6 d led to a decreased number of colony‐forming units, but returning them to broth reversed this.
Conclusions: Reproducible films of oral bacteria can be cultured in Ultrafree‐CL units.
Significance and Impact of the Study: This study has shown that Ultrafree‐CL units can be used as a simple model system to grow biofilms that could be used for dental caries research. |
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ISSN: | 1364-5072 1365-2672 |
DOI: | 10.1046/j.1365-2672.2001.01368.x |