Protective efficacy against tuberculosis of ESAT-6 secreted by a live Salmonella typhimurium vaccine carrier strain and expressed by naked DNA

We have constructed a recombinant (r) attenuated Salmonella typhimurium strain which secretes ESAT-6 of Mycobacterium tuberculosis via the hemolysin secretion system of E. coli. Additionally, we have ligated ESAT-6 to different commercially available mammalian expression systems for use as naked DNA...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Vaccine 2001-07, Vol.19 (28), p.4028-4035
Hauptverfasser:	Mollenkopf, Hans-Joachim, Groine-Triebkorn, Daniela, Andersen, Peter, Hess, Juergen, Kaufmann, Stefan H.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Bacterial - genetics Bacterial Proteins Base Sequence BCG Vaccine - genetics BCG Vaccine - pharmacology Biological and medical sciences DNA Primers - genetics Epidemiology. Vaccinations ESAT-6 ESAT-6 antigen Escherichia coli Female Fundamental and applied biological sciences. Psychology General aspects Genetic Vectors Humans Immunization, Secondary Infectious diseases Interferon-gamma - metabolism Lung - microbiology Lymphocyte Activation Medical sciences Mice Mice, Inbred BALB C Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - immunology Mycobacterium tuberculosis - isolation & purification Recombinant Proteins - genetics Salmonella typhimurium - genetics T-Lymphocytes - immunology Tuberculosis, Pulmonary - immunology Tuberculosis, Pulmonary - microbiology Tuberculosis, Pulmonary - prevention & control Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - genetics Vaccines, DNA - pharmacology Virology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We have constructed a recombinant (r) attenuated Salmonella typhimurium strain which secretes ESAT-6 of Mycobacterium tuberculosis via the hemolysin secretion system of E. coli. Additionally, we have ligated ESAT-6 to different commercially available mammalian expression systems for use as naked DNA vaccines. We studied protection against M. tuberculosis induced by vaccination with each of these constructs alone or in combination in mice. Vaccination with a single dose of r S. typhimurium secreting ESAT-6 reduced numbers of tubercle bacilli in the lungs throughout the course of infection. The combined prime-boost vaccination did not considerably enhance protection.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/S0264-410X(01)00109-8