Genetic modification of human T cells with CD20 : A strategy to purify and lyse transduced cells with anti-CD20 antibodies

A retroviral vector has been constructed that contains the human CD20 cDNA under the control of the Moloney murine leukemia virus (Mo-MuLV) LTR. Freshly isolated mononuclear cells are infected for three consecutive days in the presence of PHA and hrlL-2, and a mean 15.9% of the cells (range, 6.5 to...

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Veröffentlicht in:Human gene therapy 2000-03, Vol.11 (4), p.611-620
Hauptverfasser: INTRONA, M, BARBUI, A. M, RAMBALDI, A, BAMBACIONI, F, CASATI, C, GAIPA, G, BORLERI, G, BERNASCONI, S, BARBUI, T, GOLAY, J, BIONDI, A
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Sprache:eng
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Zusammenfassung:A retroviral vector has been constructed that contains the human CD20 cDNA under the control of the Moloney murine leukemia virus (Mo-MuLV) LTR. Freshly isolated mononuclear cells are infected for three consecutive days in the presence of PHA and hrlL-2, and a mean 15.9% of the cells (range, 6.5 to 31.7%) acquire a CD3+CD20+ phenotype. Transduced T lymphocytes grow and expand in vitro for up to 3 weeks like mock-infected cells and, as observed for the T lymphoblastoid CEM cell line, CD20 expression is maintained for several months with no change in the growth curve of the cells. CD20-expressing CEM and fresh T lymphocytes can be positively immunoselected on columns using different anti-CD20 antibodies. Exposure to monoclonal chimeric anti-CD20 IgG1(kappa) Rituximab antibody (Roche), in the presence of complement, results in effective and rapid killing of the transduced CD3+CD20+ human T cells in vitro. This approach represents a new and alternative method to gene manipulation with "suicide" genes for the production of drug-responsive T cell populations, a crucial step for the future management of graft-versus-host disease in bone marrow transplant patients.
ISSN:1043-0342
1557-7422
DOI:10.1089/10430340050015798