Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro
Objective: To develop an effective ICR mouse embryo culture medium. Design: In vitro model study. Setting: University-affiliated hospital. Animals: Four-week-old, superovulated mice. Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1). Main...
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| creator | Park, Sung E Chung, Hyung M Cha, Kwang Y Hwang, Woo S Lee, Eun S Lim, Jeong M |
| description | Objective: To develop an effective ICR mouse embryo culture medium.
Design: In vitro model study.
Setting: University-affiliated hospital.
Animals: Four-week-old, superovulated mice.
Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1).
Main Outcome Measure(s): Preimplantation development.
Result(s): In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More (
P |
| doi_str_mv | 10.1016/S0015-0282(01)01831-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70966100</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0015028201018313</els_id><sourcerecordid>70966100</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-55a6b259b1dffae8a840e7c01bed2759f761931568015cd4a5663c9019d3e7723</originalsourceid><addsrcrecordid>eNqFkV-L1DAUxYMo7uzqR1DyIKIP1dxmkrZPIsOuLiwI_nkOaXqjkTYZk7Qwn8ivaWam_nkTAgnhd869nEPIE2CvgIF8_YkxEBWr2_oFg5cMWg4Vv0c2IISshBT8Ptn8QS7IZUrfGWMSmvohuQDY8pbzdkN-Xqes-9GlbxP6TIOl2pfjJj1SnPp4CNTMY54j0nRIGSdqgs_aeee_0kVHF-a0gjmGvfOpqAfqcvm11hltDtSGuJocRbe7j3QqKqTBY2Vw_D0o0QGjW7CoPV3cEmjRnZ7F-RF5YPWY8PF6X5EvN9efd--ruw_vbndv7yojGM-VEFr2teh6GKzV2Op2y7AxDHoc6kZ0tpHQcRCyLdGYYauFlNx0DLqBY9PU_Io8P_vuY_gxY8pqcum4pPZYllYN66QExgoozqCJIaWIVu1jSS0eFDB1bEidGlLH-BUDdWpI8aJ7ug6Y-wmHv6q1kgI8WwGdjB5t1N649I-7rDkc5785Y1jSWBxGlYxDb3BwEU1WQ3D_2eQX73CwGw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70966100</pqid></control><display><type>article</type><title>Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Park, Sung E ; Chung, Hyung M ; Cha, Kwang Y ; Hwang, Woo S ; Lee, Eun S ; Lim, Jeong M</creator><creatorcontrib>Park, Sung E ; Chung, Hyung M ; Cha, Kwang Y ; Hwang, Woo S ; Lee, Eun S ; Lim, Jeong M</creatorcontrib><description>Objective: To develop an effective ICR mouse embryo culture medium.
Design: In vitro model study.
Setting: University-affiliated hospital.
Animals: Four-week-old, superovulated mice.
Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1).
Main Outcome Measure(s): Preimplantation development.
Result(s): In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More (
P<0.05) blastocysts developed after aa or aa+hb addition than after no addition, and glu addition to such medium further stimulated the formation (54%). In P-1 with aa+glu, the addition of 1 μg/mL hb was optimal. Additional improvement of blastocyst formation (78%) was achieved by ethylenediaminetetraacetic acid (EDTA), supplementation and bovine serum albumin replacement with polyvinyl alcohol (PVA) did not inhibit the development. P-1 supplemented with aa, hb, glu, EDTA, and PVA also supported the development of in vitro–derived embryos (70%).
Conclusion(s): A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(01)01831-3</identifier><identifier>PMID: 11438338</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject><![CDATA[Amino Acids - administration & dosage ; animal model ; Animals ; Biological and medical sciences ; Blastocyst - cytology ; Blastocyst - drug effects ; Blastocyst - physiology ; Culture Media - chemistry ; Culture Media - pharmacology ; Culture Techniques ; defined medium ; Drug Combinations ; Edetic Acid - administration & dosage ; Embryo, Mammalian - cytology ; Embryo, Mammalian - drug effects ; Embryo, Mammalian - metabolism ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development ; Embryonic Development ; embryotropins ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects. Development. Fetal membranes ; Glucose - administration & dosage ; Growth Substances - metabolism ; Hemoglobins - administration & dosage ; ICR mouse ; Mice ; Mice, Inbred ICR ; one-cell embryo development ; Polyvinyl Alcohol - administration & dosage ; Pregnancy ; Serum Albumin, Bovine - administration & dosage]]></subject><ispartof>Fertility and sterility, 2001-07, Vol.76 (1), p.167-174</ispartof><rights>2001 American Society for Reproductive Medicine</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-55a6b259b1dffae8a840e7c01bed2759f761931568015cd4a5663c9019d3e7723</citedby><cites>FETCH-LOGICAL-c503t-55a6b259b1dffae8a840e7c01bed2759f761931568015cd4a5663c9019d3e7723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028201018313$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1062310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11438338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sung E</creatorcontrib><creatorcontrib>Chung, Hyung M</creatorcontrib><creatorcontrib>Cha, Kwang Y</creatorcontrib><creatorcontrib>Hwang, Woo S</creatorcontrib><creatorcontrib>Lee, Eun S</creatorcontrib><creatorcontrib>Lim, Jeong M</creatorcontrib><title>Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective: To develop an effective ICR mouse embryo culture medium.
Design: In vitro model study.
Setting: University-affiliated hospital.
Animals: Four-week-old, superovulated mice.
Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1).
Main Outcome Measure(s): Preimplantation development.
Result(s): In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More (
P<0.05) blastocysts developed after aa or aa+hb addition than after no addition, and glu addition to such medium further stimulated the formation (54%). In P-1 with aa+glu, the addition of 1 μg/mL hb was optimal. Additional improvement of blastocyst formation (78%) was achieved by ethylenediaminetetraacetic acid (EDTA), supplementation and bovine serum albumin replacement with polyvinyl alcohol (PVA) did not inhibit the development. P-1 supplemented with aa, hb, glu, EDTA, and PVA also supported the development of in vitro–derived embryos (70%).
Conclusion(s): A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos.</description><subject>Amino Acids - administration & dosage</subject><subject>animal model</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>Culture Techniques</subject><subject>defined medium</subject><subject>Drug Combinations</subject><subject>Edetic Acid - administration & dosage</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development</subject><subject>Embryonic Development</subject><subject>embryotropins</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Glucose - administration & dosage</subject><subject>Growth Substances - metabolism</subject><subject>Hemoglobins - administration & dosage</subject><subject>ICR mouse</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>one-cell embryo development</subject><subject>Polyvinyl Alcohol - administration & dosage</subject><subject>Pregnancy</subject><subject>Serum Albumin, Bovine - administration & dosage</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV-L1DAUxYMo7uzqR1DyIKIP1dxmkrZPIsOuLiwI_nkOaXqjkTYZk7Qwn8ivaWam_nkTAgnhd869nEPIE2CvgIF8_YkxEBWr2_oFg5cMWg4Vv0c2IISshBT8Ptn8QS7IZUrfGWMSmvohuQDY8pbzdkN-Xqes-9GlbxP6TIOl2pfjJj1SnPp4CNTMY54j0nRIGSdqgs_aeee_0kVHF-a0gjmGvfOpqAfqcvm11hltDtSGuJocRbe7j3QqKqTBY2Vw_D0o0QGjW7CoPV3cEmjRnZ7F-RF5YPWY8PF6X5EvN9efd--ruw_vbndv7yojGM-VEFr2teh6GKzV2Op2y7AxDHoc6kZ0tpHQcRCyLdGYYauFlNx0DLqBY9PU_Io8P_vuY_gxY8pqcum4pPZYllYN66QExgoozqCJIaWIVu1jSS0eFDB1bEidGlLH-BUDdWpI8aJ7ug6Y-wmHv6q1kgI8WwGdjB5t1N649I-7rDkc5785Y1jSWBxGlYxDb3BwEU1WQ3D_2eQX73CwGw</recordid><startdate>20010701</startdate><enddate>20010701</enddate><creator>Park, Sung E</creator><creator>Chung, Hyung M</creator><creator>Cha, Kwang Y</creator><creator>Hwang, Woo S</creator><creator>Lee, Eun S</creator><creator>Lim, Jeong M</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010701</creationdate><title>Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro</title><author>Park, Sung E ; Chung, Hyung M ; Cha, Kwang Y ; Hwang, Woo S ; Lee, Eun S ; Lim, Jeong M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-55a6b259b1dffae8a840e7c01bed2759f761931568015cd4a5663c9019d3e7723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acids - administration & dosage</topic><topic>animal model</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>Culture Media - chemistry</topic><topic>Culture Media - pharmacology</topic><topic>Culture Techniques</topic><topic>defined medium</topic><topic>Drug Combinations</topic><topic>Edetic Acid - administration & dosage</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development</topic><topic>Embryonic Development</topic><topic>embryotropins</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Glucose - administration & dosage</topic><topic>Growth Substances - metabolism</topic><topic>Hemoglobins - administration & dosage</topic><topic>ICR mouse</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>one-cell embryo development</topic><topic>Polyvinyl Alcohol - administration & dosage</topic><topic>Pregnancy</topic><topic>Serum Albumin, Bovine - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sung E</creatorcontrib><creatorcontrib>Chung, Hyung M</creatorcontrib><creatorcontrib>Cha, Kwang Y</creatorcontrib><creatorcontrib>Hwang, Woo S</creatorcontrib><creatorcontrib>Lee, Eun S</creatorcontrib><creatorcontrib>Lim, Jeong M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sung E</au><au>Chung, Hyung M</au><au>Cha, Kwang Y</au><au>Hwang, Woo S</au><au>Lee, Eun S</au><au>Lim, Jeong M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2001-07-01</date><risdate>2001</risdate><volume>76</volume><issue>1</issue><spage>167</spage><epage>174</epage><pages>167-174</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective: To develop an effective ICR mouse embryo culture medium.
Design: In vitro model study.
Setting: University-affiliated hospital.
Animals: Four-week-old, superovulated mice.
Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1).
Main Outcome Measure(s): Preimplantation development.
Result(s): In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More (
P<0.05) blastocysts developed after aa or aa+hb addition than after no addition, and glu addition to such medium further stimulated the formation (54%). In P-1 with aa+glu, the addition of 1 μg/mL hb was optimal. Additional improvement of blastocyst formation (78%) was achieved by ethylenediaminetetraacetic acid (EDTA), supplementation and bovine serum albumin replacement with polyvinyl alcohol (PVA) did not inhibit the development. P-1 supplemented with aa, hb, glu, EDTA, and PVA also supported the development of in vitro–derived embryos (70%).
Conclusion(s): A modified P-1 medium was developed, and it supported the development of both in vivo- and in vitro-derived ICR mouse embryos.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11438338</pmid><doi>10.1016/S0015-0282(01)01831-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 2001-07, Vol.76 (1), p.167-174 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_70966100 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acids - administration & dosage animal model Animals Biological and medical sciences Blastocyst - cytology Blastocyst - drug effects Blastocyst - physiology Culture Media - chemistry Culture Media - pharmacology Culture Techniques defined medium Drug Combinations Edetic Acid - administration & dosage Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryo, Mammalian - metabolism Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development Embryonic Development embryotropins Female Fertilization in Vitro Fundamental and applied biological sciences. Psychology General aspects General aspects. Development. Fetal membranes Glucose - administration & dosage Growth Substances - metabolism Hemoglobins - administration & dosage ICR mouse Mice Mice, Inbred ICR one-cell embryo development Polyvinyl Alcohol - administration & dosage Pregnancy Serum Albumin, Bovine - administration & dosage |
| title | Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro |
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